Rat IL-6(Interleukin 6) ELISA Kit
中文名称：大鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 12.5-800 pg/mL
- 灵敏度： 7.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-6会与包被抗体结合。后依次加入生物素化的抗大鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-6抗体与结合在包被抗体上的大鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 12.5-800 pg/mL |
| 灵敏度 | 7.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Nano NiO induced liver toxicity via activating the NF-κB signaling pathway in rats
IF: 1.969 -
Protective effect of diethylcarbamazine inhibits NF-κB activation in isoproterenol-induced acute myocardial infarction rat model through the PARP pathway
IF: 1.692 -
Neuroprotective effects of curcumin alleviate lumbar intervertebral disc degeneration through regulating the expression of iNOS, COX?2, TGF?β1/2, MMP?9 and BDNF in a rat model
IF: 1.692Journal： Molecular Medicine Reports (2017)PMID：28901458Reactivity：RatSample Type：intervertebral discs -
Neuroprotective effect of tanshinone IIA weakens spastic cerebral palsy through inflammation, p38MAPK and VEGF in neonatal rats
IF: 1.692 -
Epigallocatechin Gallate Attenuates Hip Fracture-Induced Acute Lung Injury by Limiting Mitochondrial DNA (mtDNA) Release
IF: 1.585Journal： MEDICAL SCIENCE MONITOR (2017)PMID：28698540Reactivity：RatSample Type：Bronchoalveolar Lavage Fluid -
Angiotensin-(1-7) relieved renal injury induced by chronic intermittent hypoxia in rats by reducing inflammation, oxidative stress and fibrosis
IF: 1.578Journal： BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH (2017)PMID：28076452Reactivity：RatSample Type：Plasma -
Mitochondrial DNA-Induced Inflammatory Responses and Lung Injury in Thermal Injury Rat ModelProtective Effect of Epigallocatechin Gallate
IF: 1.349 -
Tocotrienol alleviates inflammation and oxidative stress in a rat model of spinal cord injury via suppression of transforming growth factor-β
IF: 1.261Journal： Experimental and Therapeutic Medicine (2017)PMID：28672950Reactivity：RatSample Type：Serum -
Astragalus polysaccharides combined with ibuprofen exhibit a therapeutic effect on septic rats via an anti?inflammatory cholinergic pathway
IF: 1.261Journal： Experimental and Therapeutic Medicine (2017)PMID：28912862Reactivity：RatSample Type：Plasma -
Gambogic acid exerts cardioprotective effects in a rat model of acute myocardial infarction through inhibition of inflammation, iNOS and NF?κB/p38 pathway
IF: 1.261Journal： Experimental and Therapeutic Medicine (2017)PMID：29434760Reactivity：RatSample Type：Serum
实验操作视频
