Rat IL-6(Interleukin 6) ELISA Kit
中文名称：大鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 12.5-800 pg/mL
- 灵敏度： 7.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-6会与包被抗体结合。后依次加入生物素化的抗大鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-6抗体与结合在包被抗体上的大鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 12.5-800 pg/mL |
| 灵敏度 | 7.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Cinnamaldehyde ameliorates LPS-induced cardiac dysfunction via TLR4-NOX4 pathway: The regulation of autophagy and ROS production
IF: 4.874Journal： JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (2016)PMID：27838370Reactivity：RatSample Type：Serum -
Hyperbaric Oxygen Intervention Modulates Early Brain Injury after Experimental Subarachnoid Hemorrhage in Rats: Possible Involvement of TLR4/NF-κ B-Mediated Signaling Pathway
IF: 4.652Journal： CELLULAR PHYSIOLOGY AND BIOCHEMISTRY (2016)PMID：27197977Reactivity：RatSample Type：Tissue homogenate -
Small Molecule Inhibiting Nuclear Factor-kB Ameliorates Oxidative Stress and Suppresses Renal Inflammation in Early Stage of Alloxan-Induced Diabetic Nephropathy in Rat
IF: 3.097Journal： BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY (2016)PMID：27888584Reactivity：RatSample Type：Tissue homogenate -
Venenum Bufonis induces rat neuroinflammation by activiating NF-κB pathway and attenuation of BDNF
IF: 3.055Journal： JOURNAL OF ETHNOPHARMACOLOGY (2016)PMID：27013094Reactivity：RatSample Type：Tissue homogenate -
EFFECT OF INTRAPERITONEAL RESUSCITATION WITH DIFFERENT CONCENTRATIONS OF SODIUM PYRUVATE ON INTESTINAL ISCHEMIA REPERFUSION INJURY IN HEMORRHAGIC SHOCK RAT
IF: 3.048 -
The protective role of neuregulin-1: A potential therapy for sepsis-induced cardiomyopathy
IF: 2.730 -
SIRT1 exerts neuroprotective effects by attenuating cerebral ischemia/reperfusion-induced injury via targeting p53/microRNA-22
IF: 2.348Journal： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2016)PMID：27878231Reactivity：RatSample Type：Tissue homogenate -
Anti-oxidant and anti-inflammatory effects of apigenin in a rat model of sepsis: an immunological, biochemical, and histopathological study
IF: 1.617Journal： IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2016)PMID：27144896Reactivity：RatSample Type：Serum -
Effect of apelin hormone on renal ischemia/reperfusion induced oxidative damage in rats
IF: 0.875 -
Toll-like receptor 7 involves the injury in acute kidney ischemia/reperfusion of STZ-induced diabetic rats
IF: 0.580
实验操作视频
