Rat IL-6(Interleukin 6) ELISA Kit
中文名称：大鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 12.5-800 pg/mL
- 灵敏度： 7.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-6会与包被抗体结合。后依次加入生物素化的抗大鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-6抗体与结合在包被抗体上的大鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 12.5-800 pg/mL |
| 灵敏度 | 7.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Polydatin Alleviates Small Intestine Injury during Hemorrhagic Shock as a SIRT1 Activator
IF: 3.516Journal： Oxidative Medicine and Cellular Longevity (2015)PMID：26301045Reactivity：RatSample Type：Serum -
Transplantation of Induced Pluripotent Stem Cells Alleviates Cerebral Inflammation and Neural Damage in Hemorrhagic Stroke
IF: 3.234 -
High fat diet-induced inflammation and oxidative stress are attenuated by N-acetylneuraminic acid in rats
IF: 2.763 -
Edible Bird’s Nest attenuates high fat diet-induced oxidative stress and inflammation via regulation of hepatic antioxidant and inflammatory genes
IF: 2.020Journal： BMC Complementary and Alternative Medicine (2015)PMID：26341858Reactivity：RatSample Type：Serum -
Candida glabrata induced infection of rat tracheal epithelial cells is mediated by TLR-2 induced activation of NF-κB
IF: 1.794 -
Asiaticoside attenuates the effects of spinal cord injury through antioxidant and anti?inflammatory effects, and inhibition of the p38?MAPK mechanism
IF: 1.554 -
Effects and mechanisms of Geniposide on rats with adjuvant arthritis
IF: 2.711Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2014)PMID：24583144Reactivity：RatSample Type：Cell lysate -
Anti-inflammatory effects and pharmacokinetics study of geniposide on rats with adjuvant arthritis
IF: 2.711Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2014)PMID：25434608Reactivity：RatSample Type：Cell culture supernatant -
Isoproterenol?mediated heme oxygenase?1 induction inhibits high mobility group box 1 protein release and protects against rat myocardial ischemia/reperfusion injury in vivo
IF: 1.484Journal： Molecular Medicine Reports (2014)PMID：24604346Reactivity：RatSample Type：myocardial tissues -
Dobutamine-mediated heme oxygenase-1 induction via PI3K and p38 MAPK inhibits high mobility group box 1 protein release and attenuates rat myocardial ischemia/reperfusion injury in vivo
IF: 2.018
实验操作视频
