Rat IL-6(Interleukin 6) ELISA Kit
中文名称：大鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 12.5-800 pg/mL
- 灵敏度： 7.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-6会与包被抗体结合。后依次加入生物素化的抗大鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-6抗体与结合在包被抗体上的大鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 12.5-800 pg/mL |
| 灵敏度 | 7.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
The effects of obesity on thyroid function in a metabolically healthy high-fat, high-carbohydrate diet-induced obese rat model
IF: 3.9Journal： Frontiers in Endocrinology (2025)Reactivity：RatSample Type：plasma -
Astragaloside IV attenuates cadmium induced nephrotoxicity in rats by activating Nrf2
IF: 3.8Journal： Scientific Reports (2025)Reactivity：RatSample Type：kidney tissue,serum -
Shikimic acid protects against doxorubicin-induced cardiotoxicity in rats
IF: 3.8 -
Memantine abrogates testicular dysfunction induced by risperidone in rats with a potential role of ERK1/2-Nrf2-caspase-3 signaling pathway
IF: 3.8Journal： Scientific Reports (2025)Reactivity：RatSample Type：testicular tissue -
Chemical Characterization and Green Formulation of Silver Nanoparticles by Trachyspermum?copticum L for the Treatment of Dextran Sulfate Sodium-Induced Acute Enteritis in Sprague–Dawley Rats
IF: 3.7Journal： APPLIED ORGANOMETALLIC CHEMISTRY (2025)Reactivity：RatSample Type：serum -
ADAM17/ACE2 interaction mediates cadmium-induced brain damage and neuroinflammation in Wistar rats
IF: 3.7Journal： CYTOKINE (2025) -
MiR-340-5p alleviates AECOPD by targeting MAP3K2 via Qingjin Huatan Decoction therapy
IF: 3.6Journal： JOURNAL OF LEUKOCYTE BIOLOGY (2025)Reactivity：RatSample Type：lung tissue,bronchoalveolar lavage fluid -
Macrophages‐derived small extracellular vesicles regulate chondrocyte proliferation and affect osteoarthritis progression via upregulating Osteopontin expression
IF: 3.6Journal： Journal of Cell Communication and Signaling (2025)Reactivity：RatSample Type：knee joint cartilage tissue -
Polydatin Mitigates Lead-Induced Nephropathy by Modulating Oxidative Stress, Inflammation, and the AMPK/AKT/Nrf2 Pathway in Rats
IF: 3.4Journal： BIOLOGICAL TRACE ELEMENT RESEARCH (2025)Reactivity：RatSample Type：renal tissue -
Methyltransferase-like enzyme 14 exacerbates retinal ganglion cell damage and diabetic retinopathy through N6-methyladenosine-dependent upregulation of pleckstrin homology domain and leucine rich repeat protein phosphatase 2
IF: 3.3Journal： TOXICOLOGY AND APPLIED PHARMACOLOGY (2025)Reactivity：RatSample Type：Retinal tissue
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