Rat IL-6(Interleukin 6) ELISA Kit
中文名称：大鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 12.5-800 pg/mL
- 灵敏度： 7.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的大鼠IL-6会与包被抗体结合。后依次加入生物素化的抗大鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗大鼠IL-6抗体与结合在包被抗体上的大鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 12.5-800 pg/mL |
| 灵敏度 | 7.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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The protective effects of tectoridin on bone fractures against oxidative stress via the inhibition of NF-κB and apoptotic pathways in ovariectomized rats
IF: 3.3Journal： TOXICOLOGY AND APPLIED PHARMACOLOGY (2025)Reactivity：RatSample Type：serum -
Modulation of PI3K/AKT signaling and DFT modeling via selected pharmaceutical compounds attenuates carrageenan-induced inflammation and oxidative stress in rats
IF: 3.1Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2025)Reactivity：RatSample Type：paw tissue -
Impact of lisinopril on cisplatin-induced inflammation, oxidative stress, apoptosis, and impaired steroidogenesis in rat testis: involvement of Nrf2/Keap1/HO-1 and PPARγ signaling
IF: 3.1Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2025)Reactivity：RatSample Type：teste tissue -
Modulation of AMPK by esomeprazole and canagliflozin mitigates methotrexate-induced hepatotoxicity: involvement of MAPK/JNK/ERK, JAK1/STAT3, and PI3K/Akt signaling pathways
IF: 3.1Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2025)Reactivity：RatSample Type：liver tissue -
Dapagliflozin ameliorates kidney injury following limb ischemia-reperfusion via the AMPK/SIRT1/NLRP3 pathway
IF: 3Journal： RENAL FAILURE (2025)Reactivity：RatSample Type：serum,kidney tissue -
IL-34 aggravates myocardial ischemia-reperfusion injury by upregulating the HMGB1-IL-17A-IL-6 axis through the JAK signaling pathway
IF: 2.9Journal： PLoS One (2025)Reactivity：RatSample Type：myocardial tissue -
The effects of the GLP1 analog liraglutide on allodynia and motor coordination in peripheral neuropathy induced by a chemotherapeutic agent, cisplatin
IF: 2.9Journal： JOURNAL OF MOLECULAR HISTOLOGY (2025)Reactivity：RatSample Type：dorsal root ganglia (DRG) tissue,sciatic nerves (SN) tissue -
Linc00963 up-regulation alleviates postmenopausal osteoporosis through suppression of miR-506-3p
IF: 2.8Journal： Journal of Orthopaedic Surgery and Research (2025)Reactivity：RatSample Type：serum -
Obacunone acts as a histone deacetylase 1 inhibitor to limit p38MAPK signaling and alleviate osteoarthritis progression
IF: 2.8Journal： Journal of Orthopaedic Surgery and Research (2025)Reactivity：RatSample Type：serum -
Rhynchophylline promotes microglia phenotypic transformation and repair of cerebral ischaemic injury through the JAK2/STAT3 pathway
IF: 2.7Journal： HUMAN & EXPERIMENTAL TOXICOLOGY (2025)Reactivity：RatSample Type：brain tissue
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