Rat INS(Insulin) ELISA Kit
中文名称：大鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的大鼠INS会与包被抗体结合。后依次加入生物素化的抗大鼠INS抗体和辣根过氧化物酶标记的亲和素，抗大鼠INS抗体与结合在包被抗体上的大鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 细胞培养上清、组织匀浆上清 |
| 特异性 | 可检测样本中的大鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Anthocyanin-rich black wheat as a functional food for managing type 2 diabetes mellitus: a study on high fat diet-streptozotocin-induced diabetic rats
IF: 5.100 -
Pregestational Prediabetes Induces Maternal Hypothalamic–Pituitary–Adrenal (HPA) Axis Dysregulation and Results in Adverse Foetal Outcomes
IF: 4.900Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity：RatSample Type：Plasma -
Nicotinamide Mononucleotide (NMN) Ameliorates Free Fatty Acid-Induced Pancreatic β-Cell Dysfunction via the NAD+/AMPK/SIRT1/HIF-1α Pathway
IF: 4.900Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity：Rat -
Alpha-amylase and alpha-glucosidase upregulated glucose homeostasis in high-fat fed wistar rats supplemented with cocoa flavonoid-rich aqueous
IF: 4.800 -
RANKL/RANK contributes to the pathological process of type 2 diabetes mellitus through TRAF3 activation of NIK
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：RatSample Type：Pancreatic tissue -
Capsinoids Increase Antioxidative Enzyme Activity and Prevent Obesity-Induced Cardiac Injury without Positively Modulating Body Fat Accumulation and Cardiac Oxidative Biomarkers
IF: 4.800 -
Biomarker screening using integrated bioinformatics for the development of “normal—impaired glucose intolerance—type 2 diabetes?mellitus”
IF: 4.600 -
Effects of Gossypetin on Glucose Homeostasis in Diet-Induced Pre-Diabetic Rats
IF: 4.200 -
Tyrosine nitration of glucagon impairs its function: Extending the role of heme in T2D pathogenesis
IF: 3.900 -
NLRP3 Inhibitor Alleviates Glycemic Variability-Induced Cognitive Impairment in Aged Rats with Type 2 Diabetes Mellitus
IF: 3.800Journal： MOLECULAR AND CELLULAR ENDOCRINOLOGY (2024)Reactivity：RatSample Type：serum
实验操作视频
