Rat INS(Insulin) ELISA Kit
中文名称：大鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的大鼠INS会与包被抗体结合。后依次加入生物素化的抗大鼠INS抗体和辣根过氧化物酶标记的亲和素，抗大鼠INS抗体与结合在包被抗体上的大鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 细胞培养上清、组织匀浆上清 |
| 特异性 | 可检测样本中的大鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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The effects of β-escin on inflammation, oxidative stress and Langerhans islet cells in high-fat diet and streptozotocin injection induced experimental type-2 diabetes model
IF: 1.653 -
Intraductal botulinum toxin injection suppressed the inflammation in experimental acute pancreatitis.
IF: 0.894Journal： Ulusal Travma ve Acil Cerrahi Dergisi-Turkish Journal of Trauma & Emergency Surgery (2022)PMID：36453789Reactivity：RatSample Type：blood -
Lycium barbarum polysaccharide combined with aerobic exercise ameliorated nonalcoholic fatty liver disease through restoring gut microbiota, intestinal barrier and inhibiting hepatic inflammation
IF: 6.953Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)PMID：33992651Reactivity：RatSample Type：Serum -
GLP-1-Induced AMPK Activation Inhibits PARP-1 and Promotes LXR-Mediated ABCA1 Expression to Protect Pancreatic β-Cells Against Cholesterol-Induced Toxicity Through Cholesterol Efflux
IF: 6.684 -
Curcumin in Combination with Aerobic Exercise Improves Follicular Dysfunction via Inhibition of the Hyperandrogen-Induced IRE1α/XBP1 Endoplasmic Reticulum Stress Pathway in PCOS-Like Rats
IF: 6.543Journal： Oxidative Medicine and Cellular Longevity (2021)PMID：34987702Reactivity：RatSample Type：serum -
D-ribose-L-cysteine prevents oxidative stress and cardiometabolic syndrome in high fructose high fat diet fed rats
IF: 6.530 -
Potential mechanistic pathways underlying intestinal and hepatic effects of kefir in high-fructose-fed rats
IF: 6.475Journal： FOOD RESEARCH INTERNATIONAL (2021)PMID：33992387Reactivity：ratSample Type：Tissue homogenate -
Development and Evaluation of Curcumin Encapsulated Self-assembled Nanoparticles as Potential Remedial Treatment for PCOS in a Female Rat Model
IF: 6.400Journal： International Journal of Nanomedicine (2021)PMID：34531655Reactivity：RatSample Type：serum -
Hepatoprotective Effect of Bee Bread in Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) Rats: Impact on Oxidative Stress and Inflammation
IF: 6.313 -
Effects of High Intakes of Fructose and Galactose, with or without Added Fructooligosaccharides, on Metabolic Factors, Inflammation, and Gut Integrity in a Rat Model
IF: 5.820
实验操作视频
