Rat INS(Insulin) ELISA Kit
中文名称：大鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的大鼠INS会与包被抗体结合。后依次加入生物素化的抗大鼠INS抗体和辣根过氧化物酶标记的亲和素，抗大鼠INS抗体与结合在包被抗体上的大鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 细胞培养上清、组织匀浆上清 |
| 特异性 | 可检测样本中的大鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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CGA ameliorates cognitive decline by regulating the PI3K/AKT signaling pathway and neurotransmitter systems in rats with multi-infarct dementia
IF: 1.785Journal： Experimental and Therapeutic Medicine (2020)PMID：32963600Reactivity：RatSample Type：Tissue homogenate -
Cognitive impairment by non-insulin-dependent diabetes mellitus was attenuated by dietary supplements of marble vine (Dioclea reflexa) and plantain (Musa paradisiaca) dough meals in albino rats
IF: 1.662 -
Myrothamnus flabellifolius attenuates streptozotocin-high energy diet-induced type 2 diabetes in male sprague dawley rats
Journal： Journal of Medicinal Plants Research (2020)Reactivity：RatSample Type：Plasma -
Treatment with adipose tissue-derived mesenchymal stem cells exerts anti-diabetic effects, improves long-term complications, and attenuates inflammation in type 2 diabetic rats
IF: 4.627 -
Liraglutide Ameliorates Hyperhomocysteinemia-Induced Alzheimer-Like Pathology and Memory Deficits in Rats via Multi-molecular Targeting
IF: 4.246 -
Orphan G-protein coupled receptor 183 (GPR183) potentiates insulin secretion and prevents glucotoxicity-induced β-cell dysfunction
IF: 3.693 -
Antiobesity Effect of Flaxseed Polysaccharide via Inducing Satiety due to Leptin Resistance Removal and Promoting Lipid Metabolism through the AMP-Activated Protein Kinase (AMPK) Signaling Pathway
IF: 3.571Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2019)PMID：31199141Reactivity：RatSample Type：serum -
Effects of noopept on cognitive functions and pubertal process in rats with diabetes
IF: 3.448 -
Inhibition of JAK-STAT and NF-κB signalling systems could be a novel therapeutic target against insulin resistance and type 2 diabetes
IF: 3.448 -
Insulin Resistance–Related Proteins Are Overexpressed in Patients and Rats Treated With Olanzapine and Are Reverted by Pueraria in the Rat Model
IF: 2.977Journal： JOURNAL OF CLINICAL PSYCHOPHARMACOLOGY (2019)PMID：30946280Reactivity：HumanSample Type：Plasma
实验操作视频
