Rat INS(Insulin) ELISA Kit
中文名称：大鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的大鼠INS会与包被抗体结合。后依次加入生物素化的抗大鼠INS抗体和辣根过氧化物酶标记的亲和素，抗大鼠INS抗体与结合在包被抗体上的大鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 细胞培养上清、组织匀浆上清 |
| 特异性 | 可检测样本中的大鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Green tea polyphenols attenuate hepatic steatosis, and reduce insulin resistance and inflammation in high-fat diet-induced rats
IF: 2.928Journal： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2019)PMID：31364723Reactivity：RatSample Type：blood -
GNAS gene is an important regulator of insulin secretory capacity in pancreatic β-cells
IF: 2.638 -
Abnormal expression of HSP70 may contribute to PCOS pathology
IF: 2.469 -
The Beneficial Effects of Stingless Bee Honey from Heterotrigona itama against Metabolic Changes in Rats Fed with High-Carbohydrate and High-Fat Diet
IF: 2.468Journal： International Journal of Environmental Research and Public Health (2019)PMID：31817937Reactivity：RatSample Type：Serum -
High fat-low protein diet induces metabolic alterations and cognitive dysfunction in female rats
IF: 2.411 -
Cafeteria diet increased adiposity in comparison to high fat diet in young male rats
IF: 2.353 -
Evaluating the effects of Juglans regia L. extract on hyperglycaemia and insulin sensitivity in experimental type 2 diabetes in rat
IF: 2.110Journal： ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2019)PMID：31545080Reactivity：RatSample Type：Plasma -
Reduced Expression of Chl1 gene Impairs Insulin Secretion by Down-Regulating the Expression of Key Molecules of β-cell Function
IF: 1.927Journal： EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES (2019)PMID：31614370Reactivity：RatSample Type：Cell culture supernatant -
RORB and RORC associate with human islet dysfunction and inhibit insulin secretion in INS-1 cells
IF: 1.879 -
Pterocarpus santalinus ameliorates streptozotocin-induced diabetes mellitus via anti-inflammatory pathways and enhancement of insulin function
IF: 1.854Journal： Iranian Journal of Basic Medical Sciences (2019)PMID：31579450Reactivity：RatSample Type：serum
实验操作视频
