Rat INS(Insulin) ELISA Kit
中文名称：大鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的大鼠INS会与包被抗体结合。后依次加入生物素化的抗大鼠INS抗体和辣根过氧化物酶标记的亲和素，抗大鼠INS抗体与结合在包被抗体上的大鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 细胞培养上清、组织匀浆上清 |
| 特异性 | 可检测样本中的大鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Aqueous leaf extract of Clinacanthus nutans improved metabolic indices and sorbitol‐related complications in type II diabetic rats (T2D)
IF: 1.747Journal： Food Science & Nutrition (2019)DOI：10.1002/fsn3.988PMID：31024722Reactivity：RatSample Type：Tissue homogenate,Serum -
Moringa oleifera Lam. prevents the development of high fructose diet-induced fatty liver
IF: 1.504Journal： SOUTH AFRICAN JOURNAL OF BOTANY (2019)Reactivity：RatSample Type：Serum -
Orthosiphon stamineus as a potential antidiabetic drug in maternal hyperglycemia in streptozotocin-induced diabetic rats
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Adverse effects of noise stress on glucose homeostasis and insulin resistance in Sprague-Dawley rats
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The protective effect of neonatal oral administration of oleanolic acid against the subsequent development of fructose-induced metabolic dysfunction in male and female rats
IF: 3.483 -
Tangganjian decoction ameliorates type 2 diabetes mellitus and nonalcoholic fatty liver disease in rats by activating the IRS/PI3K/AKT signaling pathway
IF: 3.457 -
Role of simvastatin and insulin in memory protection in a rat model of diabetes mellitus and dementia
IF: 3.440 -
The Chinese medicine Chai Hu Li Zhong Tang protects against non-alcoholic fatty liver disease by activating AMPKα
IF: 2.899Journal： BIOSCIENCE REPORTS (2018)PMID：30291215Reactivity：RatSample Type：Cell culture supernatant -
Mitochondria-targeted antioxidant therapy for an animal model of PCOS-IR
IF: 2.784Journal： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2018)PMID：30431108Reactivity：RatSample Type：Serum -
The pectin–insulin patch application prevents the onset of peripheral neuropathy-like symptoms in streptozotocin-induced diabetic rats
IF: 2.210Journal： CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2018)PMID：30326192Reactivity：RatSample Type：Plasma
实验操作视频
