Rat INS(Insulin) ELISA Kit
中文名称：大鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的大鼠INS会与包被抗体结合。后依次加入生物素化的抗大鼠INS抗体和辣根过氧化物酶标记的亲和素，抗大鼠INS抗体与结合在包被抗体上的大鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 细胞培养上清、组织匀浆上清 |
| 特异性 | 可检测样本中的大鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Comparison of the anti-diabetic effects of various grain and legume extracts in high-fat diet and streptozotocin-nicotinamide-induced diabetic rats
IF: 3.400 -
Aqueous extract of Peristrophe bivalvis (L.) Merr. leaf reversed the detrimental effects of nitric oxide synthase inhibitor on blood lipid profile and glucose level
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Protective effects of ghrelin on pancreas in fructose diet and streptozotocin-induced diabetic rats
IF: 2.900Journal： JOURNAL OF MOLECULAR HISTOLOGY (2024)Reactivity：RatSample Type：Serum,pancreatic tissue -
Establishing a Female Animal Model of Prediabetes Using a High-Carbohydrate, High-Fat Diet
IF: 2.800Journal： CURRENT ISSUES IN MOLECULAR BIOLOGY (2024)Reactivity：RatSample Type：plasma -
Erythrina lysistemon Hutch. abates deranged metabolic indices linked with diabetic complications in experimental rat model
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Impact of L-Arginine on diabetes-induced neuropathy and myopathy: Roles of PAI-1, Irisin, oxidative stress, NF-κβ, autophagy and microRNA-29a
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Spirulina versus metformin for controlling some insulin signaling pathway genes in induced polycystic ovary syndrome rat model
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Antidiabetic Effect of Acalypha hispida Extract and Nanoextract: Mechanism of Action on Pancreatic β-Cell Function via Histone Acetylation
IF: 2.100Journal： Advances in Pharmacological and Pharmaceutical Sciences (2024)Reactivity：RatSample Type：Serum -
Visseral Lipectomy Improves Metabolic Syndrome Parameters and Adipokines in a Rat Model of Metabolic Syndrome Induced by Monosodium Glutamate
IF: 2.000Journal： AESTHETIC PLASTIC SURGERY (2024)Reactivity：RatSample Type：serum -
Role of toll-like receptor 4/mutant myeloid differentiation primary response 88/nuclear factor kappa-B mediated inflammation in diabetes mellitus with Northwest dryness syndrome
IF: 2.000Journal： Journal of Traditional Chinese Medicine (2024)Reactivity：Rat
实验操作视频
