Rat INS(Insulin) ELISA Kit
中文名称：大鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的大鼠INS会与包被抗体结合。后依次加入生物素化的抗大鼠INS抗体和辣根过氧化物酶标记的亲和素，抗大鼠INS抗体与结合在包被抗体上的大鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 细胞培养上清、组织匀浆上清 |
| 特异性 | 可检测样本中的大鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Shenling Guchang prescription ameliorates intestinal barrier inflammation in gestational diabetes rats via TLR4/NF-κB pathway
IF: 1.900Journal： Drug Discoveries and Therapeutics (2024)Reactivity：RatSample Type：Serum -
Farrerol Alleviates Diabetic Cardiomyopathy by Regulating AMPK-Mediated Cardiac Lipid Metabolic Pathways in Type 2 Diabetic Rats
IF: 1.800Journal： CELL BIOCHEMISTRY AND BIOPHYSICS (2024)Reactivity：RatSample Type：serum -
An exploratory investigation of lipid-lowering potential of spirulina (Arthrospira platensis) targeting apoprotein-E in chronic hyperlipidemic wistar albino rats
IF: 1.700Journal： Cogent Food & Agriculture (2024)Reactivity：RatSample Type：serum -
Protective role of MerTK in diabetic peripheral neuropathy via inhibition of the NF-κb signaling pathway
IF: 1.600Journal： EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES (2024)Reactivity：RatSample Type：plasma -
Histological and biochemical effects of an ethanolic extract of Myrtus communis leaf on the pancreases of rats fed high fat diets
IF: 1.600Journal： BIOTECHNIC & HISTOCHEMISTRY (2024)Reactivity：RatSample Type：serum -
Effect of irisin on ovarian phosphatidylinositol-3-kinase/protein kinase B signaling pathway and mitogen-activated protein kinase/extracellular signal-regulated kinase pathways of rats with polycystic ovary syndrome
IF: 1.600Journal： JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH (2024)Reactivity：RatSample Type：blood -
Dictamnine Reduces Gestational Diabetic Complications in Streptozotocin-Induced Diabetic Pregnant Rats via Regulating Inflammation and NOX-2, MCP-1/AGE–RAGE Pathway
IF: 1.400Journal： Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy (2024)Reactivity：RatSample Type：serum -
Diabetes disrupts osteometric and trabecular morphometric parameters in the Zucker Diabetic Sprague–Dawley rat femur
IF: 1.400 -
Exposure to fluoride exacerbates the cognitive deficit of diabetic patients living in areas with endemic fluorosis, as well as of rats with type 2 diabetes induced by streptozotocin via a mechanism that may involve excessive activation of the poly(ADP ribose) polymerase-1/P53 pathway
IF: 9.800 -
DEHP and DBP, common phthalates, induce glucose metabolism disorders in rats via oxidative damage of PI3K/Akt/GLUT4 signaling
IF: 8.900
实验操作视频
