Rat INS(Insulin) ELISA Kit
中文名称：大鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的大鼠INS会与包被抗体结合。后依次加入生物素化的抗大鼠INS抗体和辣根过氧化物酶标记的亲和素，抗大鼠INS抗体与结合在包被抗体上的大鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 细胞培养上清、组织匀浆上清 |
| 特异性 | 可检测样本中的大鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Therapeutic Potential of Selenium Nanoparticles on Letrozole-Induced Polycystic Ovarian Syndrome in Female Wistar Rats
IF: 3.900 -
Effects of Whey Protein Combined with Amylopectin/Chromium on the Muscle Protein Synthesis and mTOR Phosphorylation in Exercised Rats
IF: 3.900 -
Roflumilast ameliorates diabetic nephropathy in rats through down-regulation of JAK/STAT signaling pathway
IF: 3.600Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2023)PMID：37318524Reactivity：RatSample Type：serum -
METTL14-mediated lncRNA XIST silencing alleviates GDM progression by facilitating trophoblast cell proliferation and migration via the miR-497-5p/FOXO1 axis
IF: 3.600Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2023)PMID：38229320Reactivity：RatSample Type：serum -
PDHA1 Alleviates Myocardial Ischemia-Reperfusion Injury by Improving Myocardial Insulin Resistance During Cardiopulmonary Bypass Surgery in Rats
IF: 3.400Journal： CARDIOVASCULAR DRUGS AND THERAPY (2023)PMID：37610688Reactivity：RatSample Type：Serum,myocardial tissue -
Development of pancreatic islet cells in the extrahepatic bile ducts of rats with experimentally-induced metabolic syndrome
IF: 3.000Journal： ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2023)PMID：37651586Reactivity：RatSample Type：plasma -
Bo′s abdominal acupuncture improves disordered metabolism in obese type 2 diabetic rats through regulating fibroblast growth factor 21 and its related adipokines
IF: 2.600Journal： Journal of Traditional Chinese Medicine (2023)PMID：37946482Reactivity：RatSample Type：serum -
Mechanism of Bile-Processed Coptidis Rhizoma to Treat Nonalcoholic Fatty Liver Disease in Type 2 Diabetes Mellitus Based on UPLC-Q-TOF/MS and Network Pharmacology
IF: 2.100 -
LncRNA PWRN2 promotes polycystic ovary syndrome progression via epigenetically reducing ATRX by recruiting LSD1
IF: 2.100 -
Alleviation of adipose-hepatic glycolipid dysregulation by acetate in experimental PCOS model is associated with NF-κB/NLRP3 repression
IF: 2.100Journal： CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2023)Reactivity：RatSample Type：plasma
实验操作视频
