Rat INS(Insulin) ELISA Kit
中文名称：大鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 6.25-400 pg/mL
- 灵敏度： 3.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的大鼠INS会与包被抗体结合。后依次加入生物素化的抗大鼠INS抗体和辣根过氧化物酶标记的亲和素，抗大鼠INS抗体与结合在包被抗体上的大鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 6.25-400 pg/mL |
| 灵敏度 | 3.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆 |
| 特异性 | 可检测样本中的大鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Protective effects of ghrelin on pancreas in fructose diet and streptozotocin-induced diabetic rats
IF: 2.9Journal： JOURNAL OF MOLECULAR HISTOLOGY (2024)Reactivity：RatSample Type：Serum,pancreatic tissue -
Systemic and tissue-specific spexin response to acute treadmill exercise in rats
IF: 2.8 -
Establishing a Female Animal Model of Prediabetes Using a High-Carbohydrate, High-Fat Diet
IF: 2.8Journal： CURRENT ISSUES IN MOLECULAR BIOLOGY (2024)Reactivity：RatSample Type：plasma -
Erythrina lysistemon Hutch. abates deranged metabolic indices linked with diabetic complications in experimental rat model
IF: 2.7 -
Role of Thymoquinone on sleep restriction and its mitigating effect on leptin-mediated signaling pathway in rat brain
IF: 2.6Journal： MOLECULAR BIOLOGY REPORTS (2024)Reactivity：RatSample Type：serum -
Dehydroepiandrosterone with a high-fat diet treatment at inducing polycystic ovary syndrome in rat model
IF: 2.1 -
Antidiabetic Effect of Acalypha hispida Extract and Nanoextract: Mechanism of Action on Pancreatic β-Cell Function via Histone Acetylation
IF: 2.1Journal： Advances in Pharmacological and Pharmaceutical Sciences (2024)Reactivity：RatSample Type：Serum -
Visseral Lipectomy Improves Metabolic Syndrome Parameters and Adipokines in a Rat Model of Metabolic Syndrome Induced by Monosodium Glutamate
IF: 2.0Journal： AESTHETIC PLASTIC SURGERY (2024)Reactivity：RatSample Type：serum -
Role of toll-like receptor 4/mutant myeloid differentiation primary response 88/nuclear factor kappa-B mediated inflammation in diabetes mellitus with Northwest dryness syndrome
IF: 2.0Journal： Journal of Traditional Chinese Medicine (2024)Reactivity：Rat -
Shenling Guchang prescription ameliorates intestinal barrier inflammation in gestational diabetes rats via TLR4/NF-κB pathway
IF: 1.9Journal： Drug Discoveries and Therapeutics (2024)Reactivity：RatSample Type：Serum
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
