Rat INS(Insulin) ELISA Kit
中文名称：大鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 6.25-400 pg/mL
- 灵敏度： 3.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的大鼠INS会与包被抗体结合。后依次加入生物素化的抗大鼠INS抗体和辣根过氧化物酶标记的亲和素，抗大鼠INS抗体与结合在包被抗体上的大鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 6.25-400 pg/mL |
| 灵敏度 | 3.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆 |
| 特异性 | 可检测样本中的大鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Antidiabetic impact of novel sophorolipids produced via microbial conversion of Moringa oleifera and Lepidium sativum oil cakes utilizing locally isolated yeast strains in streptozotocin-induced diabetic wistar rats: role of glucagon-like peptide-1
IF: 0.7Journal： Egyptian Pharmaceutical Journal (2024)Reactivity：RatSample Type：serum -
Antidiabetic activity of Berberis brandisiana is possibly mediated through modulation of insulin signaling pathway, inflammatory cytokines and adipocytokines in high fat diet and streptozotocin-administered rats
IF: 5.600 -
Telmisartan versus metformin in downregulating myostatin gene expression and enhancing insulin sensitivity in the skeletal muscles of type 2 diabetic rat model
IF: 5.6 -
Pancreastatin inhibitor PSTi8 ameliorates streptozotocin-induced diabetes by suppressing hepatic glucose production
IF: 5.000 -
Chemical Constituents and Hypoglycemic Mechanisms of Dendrobium nobile in Treatment of Type 2 Diabetic Rats by UPLC-ESI-Q-Orbitrap, Network Pharmacology and In Vivo Experimental Verification
IF: 4.600 -
Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome
IF: 4.600 -
Mechanism of Xiaoyao San in treating non-alcoholic fatty liver disease with liver depression and spleen deficiency: based on bioinformatics, metabolomics and in?vivo experiments
IF: 4.4Journal： JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS (2023)PMID：37440274Reactivity：RatSample Type：serum -
6-gingerol ameliorates weight gain and insulin resistance in metabolic syndrome rats by regulating adipocytokines
IF: 4.100 -
Mango ginger (Curcuma amada Roxb.) may alleviate the effect of high-fat diet/streptozotocin-induced diabetes by activation of the GSK-3β/Fyn/Nrf2 pathway
IF: 3.9 -
Effect of Boric Acid on Metabolic Peptides and Some Biochemical Parameters in Experimental Diabetic Rats
IF: 3.9
实验操作视频
