Rat INS(Insulin) ELISA Kit
中文名称：大鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 6.25-400 pg/mL
- 灵敏度： 3.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的大鼠INS会与包被抗体结合。后依次加入生物素化的抗大鼠INS抗体和辣根过氧化物酶标记的亲和素，抗大鼠INS抗体与结合在包被抗体上的大鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 6.25-400 pg/mL |
| 灵敏度 | 3.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆 |
| 特异性 | 可检测样本中的大鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Effects of combination of obesity, diabetes, and hypoxia on inflammatory regulating genes and cytokines in rat pancreatic tissues and serum
IF: 3.061 -
Biochemical Investigation of Therapeutic Potentials of Plant-Based Bioactive Compounds as Stimulators of Glucagon like peptide-1 Secretion
IF: 2.623 -
Orally administered zingerone does not mitigate alcohol-induced hepatic oxidative stress in growing Sprague Dawley rat pups
IF: 2.597Journal： DRUG AND CHEMICAL TOXICOLOGY (2022)Reactivity：RatSample Type：plasma -
Time-Restricted Feeding Improved Vascular Endothelial Function in a High-Fat Diet-Induced Obesity Rat Model
IF: 2.518 -
AMPK/mTOR-mediated therapeutic effect of metformin on myocardial ischaemia reperfusion injury in diabetic rat
IF: 1.738 -
Pancreastatin mediated regulation of UCP-1 and energy expenditure in high fructose fed perimenopausal rats
IF: 5.037 -
Hypoglycemic and hypolipidemic effects of total glycosides of Cistanche tubulosa in diet/streptozotocin-induced diabetic rats
IF: 4.360 -
Biosynthesis of magnesium hydroxide nanomaterials using Monodora myristica, antioxidative activities and effect on disrupted glucose metabolism in streptozotocin-induced diabetic rat
IF: 4.240 -
Ezetimibe attenuates experimental diabetes and renal pathologies via targeting the advanced glycation, oxidative stress and AGE-RAGE signalling in rats
IF: 4.076Journal： ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2021)PMID：33508970Reactivity：RatSample Type：Plasma -
Variations in the mRNA expression level of UDP-GlcNAc epimerase/ManNAc kinase and neuraminidase 1 genes in organs of type 2 diabetic animals
IF: 2.916
实验操作视频
