Rat Irisin ELISA Kit
中文名称：大鼠鸢尾素(Irisin)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 78.13-5000 pg/mL
- 灵敏度： 46.88 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠Irisin抗体包被于酶标板上，实验时样品（或标准品）中的大鼠Irisin会与包被抗体结合。后依次加入生物素化的抗大鼠Irisin抗体和辣根过氧化物酶标记的亲和素，抗大鼠Irisin抗体与结合在包被抗体上的大鼠Irisin结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，Irisin浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中Irisin的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 78.13-5000 pg/mL |
| 灵敏度 | 46.88 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠Irisin，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Aerobic exercise inhibits renal EMT by promoting irisin expression in SHR
IF: 5.800 -
Chronic arsenite exposure induced skeletal muscle atrophy by disrupting angiotensin II-melatonin axis in rats
IF: 4.500 -
Exercise-induced irisin improves follicular dysfunction by inhibiting IRE1α-TXNIP/ROS-NLRP3 pathway in PCOS
IF: 4.000 -
The effects of acupuncture on depression by regulating BDNF-related balance via lateral habenular nucleus BDNF/TrkB/CREB signaling pathway in rats
IF: 2.700 -
Exercise-induced FNDC5/irisin protects nucleus pulposus cells against senescence and apoptosis by activating autophagy
IF: 12.153 -
Irisin pathways in hearts of Type 1 diabetic adult male rats following 6?weeks of moderate and high-volume aerobic exercise on a treadmill
Journal： Sport Sciences for Health (2022)Reactivity：RatSample Type：left ventricle -
Curcumin in Combination with Aerobic Exercise Improves Follicular Dysfunction via Inhibition of the Hyperandrogen-Induced IRE1α/XBP1 Endoplasmic Reticulum Stress Pathway in PCOS-Like Rats
IF: 6.543Journal： Oxidative Medicine and Cellular Longevity (2021)PMID：34987702Reactivity：RatSample Type：serum -
Irisin vs. Treadmill Exercise in Post Myocardial Infarction Cardiac Rehabilitation in Rats
IF: 1.895Journal： ARCHIVES OF MEDICAL RESEARCH (2019)PMID：31349953Reactivity：RatSample Type：serum, left ventricular cardiac , muscle
实验操作视频
