Rat KIM-1(Kidney Injury Molecule 1) ELISA Kit
中文名称：大鼠肾损伤分子1(KIM-1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠KIM-1抗体包被于酶标板上，实验时样品（或标准品）中的大鼠KIM-1会与包被抗体结合。后依次加入生物素化的抗大鼠KIM-1抗体和辣根过氧化物酶标记的亲和素，抗大鼠KIM-1抗体与结合在包被抗体上的大鼠KIM-1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，KIM-1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中KIM-1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠KIM-1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Implementing losartan potassium-laden pegylated nanocubic vesicles as a novel nanoplatform to alleviate cisplatin-induced nephrotoxicity via blocking apoptosis and activating the wnt/β-catenin/TCF-4 pathway
IF: 5.200Journal： LIFE SCIENCES (2024)Reactivity：RatSample Type：kidney tissue -
Enhanced eNOS/nitric oxide production by nebivolol interferes with TGF-β1/Smad3 signaling and collagen I deposition in the kidney after prolonged tacrolimus administration
IF: 5.200 -
Unlocking renal Restoration: Mesaconine from Aconitum plants restore mitochondrial function to halt cell apoptosis in acute kidney injury
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：RatSample Type：kidney tissue -
Nephroprotective Activity of Angelica keiskei (Miq). Koidz. on Cisplatin-Induced Rats: Reducing Serum Creatinine, Urea Nitrogen, KIM-1, and Suppressing NF-kappaB p65 and COX-2
IF: 4.700Journal： Drug Design Development and Therapy (2024)Reactivity：RatSample Type：kidney tissue -
Piperazine ferulate inhibits diabetic nephropathy by suppressing AGE/RAGE-mediated inflammatory signaling in rats and podocytes
IF: 4.400Journal： Frontiers in Pharmacology (2024)Reactivity：RatSample Type：serum -
Repurposing Dapagliflozin for Mitigation of the Kidney Injury Triggered by Cadmium in Rats: Role of Autophagy, Apoptosis, and the SIRT1/Nrf2/HO-1 Pathway
IF: 4.300 -
Sitagliptin alleviates renal steatosis and endoplasmic reticulum stress in high fat diet-induced obese rats by targeting SREBP-1/CD36 signaling pathway
IF: 4.200Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2024)Reactivity：RatSample Type：renal tissue -
Renoprotective effect of esculetin against ischemic acute kidney injury-diabetic comorbidity
IF: 3.300 -
Protective effect of deinoxanthin in sorafenib-induced nephrotoxicity in rats with the hepatocellular carcinoma model
IF: 3.100Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2024)Reactivity：RatSample Type：renal tissue -
Lycopene alleviates 5-fluorouracil-induced nephrotoxicity by modulating PPAR-γ, Nrf2/HO-1, and NF-κB/TNF-α/IL-6 signals
IF: 3.000
实验操作视频
