Rat LBP(Lipopolysaccharide Binding Protein) ELISA Kit
中文名称：大鼠脂多糖结合蛋白(LBP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 1.88-120 ng/mL
- 灵敏度： 1.13 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠LBP抗体包被于酶标板上，实验时样品（或标准品）中的大鼠LBP会与包被抗体结合。后依次加入生物素化的抗大鼠LBP抗体和辣根过氧化物酶标记的亲和素，抗大鼠LBP抗体与结合在包被抗体上的大鼠LBP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LBP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LBP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.88-120 ng/mL |
| 灵敏度 | 1.13 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠LBP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Phenylethanol Glycoside from Cistanche tubulosa Attenuates BSA-Induced Liver Fibrosis in Rats by Modulating the Gut Microbiota–Liver Axis
IF: 4.300 -
Prebiotic Potential of Goji Berry (Lycium barbarum) in Improving Intestinal Integrity and Inflammatory Profiles via Modification of the Gut Microbiota in High-Fat Diet-Fed Rats
IF: 1.700Journal： JOURNAL OF MEDICINAL FOOD (2024)Reactivity：RatSample Type：serum -
Medication overuse headache is associated with elevated lipopolysaccharide binding protein and pro-inflammatory molecules in the bloodstream
IF: 7.400 -
High dietary copper intake induces perturbations in the gut microbiota and affects host ovarian follicle development
IF: 6.800Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2023)PMID：36948015Reactivity：RatSample Type：Serum -
Modulation of Gut Microbiota and Intestinal Barrier Integrity and Inflammation Profile in High Fat-fed Rats
IF: 3.200Journal： BIOTECHNOLOGY AND BIOPROCESS ENGINEERING (2023)Reactivity：RatSample Type：serum -
Gypenosides counteract hepatic steatosis and intestinal barrier injury in rats with metabolic associated fatty liver disease by modulating the adenosine monophosphate activated protein kinase and Toll-like receptor 4/nuclear factor kappa B pathways
IF: 3.889 -
Lycium barbarum polysaccharide combined with aerobic exercise ameliorated nonalcoholic fatty liver disease through restoring gut microbiota, intestinal barrier and inhibiting hepatic inflammation
IF: 6.953Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)PMID：33992651Reactivity：RatSample Type：Plasma -
Fecal microbiota transplantation ameliorates gut microbiota imbalance and intestinal barrier damage in rats with stress-induced depressive-like behavior
IF: 3.386 -
Protective Effect of Ursolic Acid on the Intestinal Mucosal Barrier in a Rat Model of Liver Fibrosis
IF: 3.201 -
Effect of heat-killed Streptococcus thermophilus on type 2 diabetes rats
IF: 2.353
实验操作视频
