Rat LEP(Leptin) ELISA Kit
中文名称：大鼠瘦素(LEP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠LEP抗体包被于酶标板上，实验时样品（或标准品）中的大鼠LEP会与包被抗体结合。后依次加入生物素化的抗大鼠LEP抗体和辣根过氧化物酶标记的亲和素，抗大鼠LEP抗体与结合在包被抗体上的大鼠LEP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LEP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LEP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠LEP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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The cardioprotective effects of Fruitflow? against Doxorubicin-induced toxicity in rat cardiomyoblast cells H9c2 (2?1) and high-fat diet-induced dyslipidemia and pathological alteration in cardiac tissue of Wistar Albino rats
IF: 6.900Journal： BIOMEDICINE & PHARMACOTHERAPY (2024)Reactivity：RatSample Type：heart tissue -
Anthocyanin-rich black wheat as a functional food for managing type 2 diabetes mellitus: a study on high fat diet-streptozotocin-induced diabetic rats
IF: 5.100 -
Sulforaphane's role in Redefining autophagic Responses in depression associated with polycystic ovarian syndrome: Unveiling the SIRT1/AMPK/LKB1 pathway connection
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The Influence of a High-Cholesterol Diet and Forced Training on Lipid Metabolism and Intestinal Microbiota in Male Wistar Rats
IF: 4.900Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity：RatSample Type：serum -
Leptin limits hepatic lipid accumulation and inflammation via vagal activation of the JAK2-STAT3/AMPK pathway
IF: 4.800Journal： JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2024)Reactivity：RatSample Type：Serum,cerebrospinal fluid (CSF) -
Modulation of Metabolic Pathways and Protection against Cadmium-Induced Disruptions with Taxifolin-Enriched Extract
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Integrating untargeted and oxylipins-targeted metabolomics to reveal the anti-obesity and hypolipidemic mechanism of conjugated linoleic acid in high-fat diet rats
IF: 3.800Journal： Journal of Functional Foods (2024)Reactivity：RatSample Type：serum -
A Cameroon Western Regions high-fat diet (MACAPOS 2) induces visceral obesity in rat
IF: 3.400 -
Establishing a Female Animal Model of Prediabetes Using a High-Carbohydrate, High-Fat Diet
IF: 2.800Journal： CURRENT ISSUES IN MOLECULAR BIOLOGY (2024)Reactivity：RatSample Type：plasma -
Acetate abates adipose-ovarian endocrinometabolic disturbance in experimentally induced polycystic ovarian syndrome
IF: 2.100
Q1:循环瘦素和瘦素是一个指标吗？
循环瘦素就是指瘦素，循环是形容瘦素水平的循环波动
实验操作视频
