Rat LEP(Leptin) ELISA Kit
中文名称：大鼠瘦素(LEP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠LEP抗体包被于酶标板上，实验时样品（或标准品）中的大鼠LEP会与包被抗体结合。后依次加入生物素化的抗大鼠LEP抗体和辣根过氧化物酶标记的亲和素，抗大鼠LEP抗体与结合在包被抗体上的大鼠LEP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LEP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LEP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠LEP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Acetate rescues defective brain-adipose metabolic network in obese Wistar rats by modulation of peroxisome proliferator-activated receptor-γ
IF: 4.380Journal： Scientific Reports (2021)PMID：34556775Reactivity：RatSample Type：plasma, brain tissue, adipose tissue -
Bee bread mitigates downregulation of steroidogenic genes, decreased spermatogenesis, and epididymal oxidative stress in male rats fed with high-fat diet
IF: 4.310Journal： AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM (2021)PMID：34229480Reactivity：RatSample Type：serum, testis -
A comparative study between curcumin and curcumin nanoemulsion on high-fat, high-fructose diet-induced impaired spermatogenesis in rats
IF: 3.765 -
Treadmill running induces remodeling of the infrapatellar fat pad in an intensity-dependent manner
IF: 2.359Journal： Journal of Orthopaedic Surgery and Research (2021)PMID：34074301Reactivity：RatSample Type：serum -
Investigation of therapeutic potential of three endemic Cirsium species for global health problem obesity
IF: 2.315Journal： SOUTH AFRICAN JOURNAL OF BOTANY (2021)Reactivity：RatSample Type：Serum -
Short term effects of experimental gastric outlet obstruction and truncal vagotomy on gut hormones
IF: 1.718 -
Sildenafil ameliorates leptin resistance and normalizes lipid handling in the hypothalamic and adipose tissues of testosterone-exposed pregnant rats
-
Acetate supplementation restores testicular function by modulating Nrf2/PPAR-γ in high fat diet-induced obesity in Wistar rats
Journal： Journal of Diabetes and Metabolic Disorders (2021)PMID：34900819Reactivity：RatSample Type：plasma -
Athrixia phylicoides tea infusion (bushman tea) improves adipokine balance, glucose homeostasis and lipid parameters in a diet-induced metabolic syndrome rat model
Journal： BMC Complementary Medicine and Therapies (2021)Reactivity：RatSample Type：serum -
Fish oil and corn oil induced differential effect on beiging of visceral and subcutaneous white adipose tissue in high-fat-diet-induced obesity
IF: 4.873
实验操作视频
