Rat LEP(Leptin) ELISA Kit
中文名称：大鼠瘦素(LEP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠LEP抗体包被于酶标板上，实验时样品（或标准品）中的大鼠LEP会与包被抗体结合。后依次加入生物素化的抗大鼠LEP抗体和辣根过氧化物酶标记的亲和素，抗大鼠LEP抗体与结合在包被抗体上的大鼠LEP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LEP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LEP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠LEP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Effect of different levels of hydrolysable tannin intake on the reproductive hormones and serum biochemical indices in healthy female rats
IF: 3.998 -
Chronic exposure of bisphenol A impairs carbohydrate and lipid metabolism by altering corresponding enzymatic and metabolic pathways
IF: 3.292Journal： ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020)PMID：32339907Reactivity：RatSample Type：Serum -
Nicotine-mediated upregulation of microRNA-141 expression determines adipokine-intervened insulin resistance
IF: 3.292Journal： ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020)PMID：33002592Reactivity：RatSample Type：Serum -
Orlistat attenuates obesity-induced decline in steroidogenesis and spermatogenesis by up-regulating steroidogenic genes
IF: 2.860 -
Myrothamnus flabellifolius attenuates streptozotocin-high energy diet-induced type 2 diabetes in male sprague dawley rats
Journal： Journal of Medicinal Plants Research (2020)Reactivity：RatSample Type：Plasma -
Synergistic effects of the capsaicinoid nonivamide and rosuvastatin on obesity-related endothelial dysfunction in rat fed a high-fat diet
IF: 3.766Journal： PHYTOTHERAPY RESEARCH (2019)DOI：10.1002/ptr.6369PMID：31141276Reactivity：RatSample Type：blood -
Combined treatment with interleukin-1 and tumor necrosis factor-alpha antagonists improve type 2 diabetes in rats
IF: 2.210Journal： CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2018)PMID：29558631Reactivity：RatSample Type：Serum -
In utero exposure to germinated brown rice and its oryzanol-rich extract attenuated high fat diet-induced insulin resistance in F1 generation of rats
IF: 2.288Journal： BMC Complementary and Alternative Medicine (2017)PMID：28109299Reactivity：RatSample Type：Serum -
The adverse effects of chronic low-dose exposure to nonylphenol on type 2 diabetes mellitus in high sucrose-high fat diet-treated rats
IF: 2.200 -
Effect of neonatal orally administered S-allyl cysteine in high-fructose diet fed Wistar rats
IF: 2.070Journal： Journal of Developmental Origins of Health and Disease (2017)PMID：29151399Reactivity：RatSample Type：Plasma
实验操作视频
