Rat LEP(Leptin) ELISA Kit
中文名称：大鼠瘦素(LEP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠LEP抗体包被于酶标板上，实验时样品（或标准品）中的大鼠LEP会与包被抗体结合。后依次加入生物素化的抗大鼠LEP抗体和辣根过氧化物酶标记的亲和素，抗大鼠LEP抗体与结合在包被抗体上的大鼠LEP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LEP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LEP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠LEP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Perinatal exposure to germinated brown rice and its gamma amino-butyric acid-rich extract prevents high fat diet-induced insulin resistance in first generation rat offspring
IF: 3.226 -
Curcumin ameliorates high?fat diet?induced spermatogenesis dysfunction
IF: 1.559 -
Changes of adipocytokine expression after diabetic rats received sitagliptin and the molecular mechanism
IF: 0.841Journal： Asian Pacific Journal of Tropical Medicine (2016)PMID：27633305Reactivity：RatSample Type：Serum -
Combined high dose vitamin C and E increases oxidative stress and visceral fat mass in rats treated by depot-medroxyprogesterone acetate
Journal： Middle East Fertility Society Journal (2016)Reactivity：RatSample Type：serum -
Edible Bird’s Nest Prevents High Fat Diet-Induced Insulin Resistance in Rats
IF: 2.164 -
N-Acetylneuraminic acid attenuates hypercoagulation on high fat diet-induced hyperlipidemic rats
IF: 2.162 -
Protective effects of metformin on reproductive function in obese male rats induced by high-fat diet
IF: 1.718Journal： JOURNAL OF ASSISTED REPRODUCTION AND GENETICS (2015)PMID：26081124Reactivity：RatSample Type：Serum -
N-Acetylneuraminic Acid Supplementation Prevents High Fat Diet-Induced Insulin Resistance in Rats through Transcriptional and Nontranscriptional Mechanisms
IF: 1.579
实验操作视频
