Rat LH(Luteinizing Hormone) ELISA Kit
中文名称：大鼠促黄体生成激素(LH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 1.56-100 mIU/mL
- 灵敏度： 0.94 mIU/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠LH抗体包被于酶标板上，实验时样品（或标准品）中的大鼠LH会与包被抗体结合。后依次加入生物素化的抗大鼠LH抗体和辣根过氧化物酶标记的亲和素，抗大鼠LH抗体与结合在包被抗体上的大鼠LH结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LH浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LH的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 mIU/mL |
| 灵敏度 | 0.94 mIU/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠LH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Scream Sound-induced Chronic Psychological Stress Results in Diminished Ovarian Reserve in Adult Female Rat
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Testicular ultrastructure and hormonal changes following administration of tenofovir disoproxil fumarate-loaded silver nanoparticle in type-2 diabetic rats
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Moderate Aerobic Exercise Regulates Follicular Dysfunction by Initiating Brain-Derived Neurotrophic Factor (BDNF)-Mediated Anti-Apoptotic Signaling Pathways in Polycystic Ovary Syndrome
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Long-Term Wi-Fi Exposure From Pre-Pubertal to Adult Age on the Spermatogonia Proliferation and Protective Effects of Edible Bird’s Nest Supplementation
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The effects of prenatal and lactational bisphenol A and/or di(2-ethylhexyl) phthalate exposure on female reproductive system
IF: 4.019Journal： TOXICOLOGY MECHANISMS AND METHODS (2022)Reactivity：RatSample Type：Plasma -
Spirulina therapeutic potentiality in polycystic ovarian syndrome management using DHEA-induced rat model
IF: 3.784Journal： European Review for Medical and Pharmacological Sciences (2022)PMID：35503619Reactivity：RatSample Type：serum -
A Selective Histamine H4 Receptor Antagonist, JNJ7777120, Role on glutamate Transporter Activity in Chronic Depression
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Effects of boric acid and potassium metaborate on cytokine levels and redox stress parameters in a wound model infected with methicillin?resistant Staphylococcus aureus
IF: 3.423 -
Bisphenol A exposure advances puberty onset by changing Kiss1 expression firstly in arcuate nucleus at juvenile period in female rats
IF: 3.421 -
Role and mechanism of the p-JAK2/p-STAT3 signaling pathway in follicular development in PCOS rats
IF: 3.255Journal： GENERAL AND COMPARATIVE ENDOCRINOLOGY (2022)PMID：36202220Reactivity：RatSample Type：serum
实验操作视频
