Rat LH(Luteinizing Hormone) ELISA Kit
中文名称：大鼠促黄体生成激素(LH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 1.56-100 mIU/mL
- 灵敏度： 0.94 mIU/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠LH抗体包被于酶标板上，实验时样品（或标准品）中的大鼠LH会与包被抗体结合。后依次加入生物素化的抗大鼠LH抗体和辣根过氧化物酶标记的亲和素，抗大鼠LH抗体与结合在包被抗体上的大鼠LH结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LH浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LH的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 mIU/mL |
| 灵敏度 | 0.94 mIU/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠LH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Edible Bird’s Nest Supplementation Improves Male Reproductive Parameters of Sprague Dawley Rat
IF: 5.811 -
Icariin Ameliorates Diabetic Renal Tubulointerstitial Fibrosis by Restoring Autophagy via Regulation of the miR-192-5p/GLP-1R Pathway
IF: 5.811 -
Paeoniflorin attenuates DHEA-induced polycystic ovary syndrome via inactivation of TGF-β1/Smads signaling pathway in vivo
IF: 5.682 -
Effects of vancomycin linoleic acid nanoparticles on male reproductive indices of Sprague–Dawley rats
IF: 5.678Journal： Artificial Cells Nanomedicine and Biotechnology (2021)PMID：34425727Reactivity：RatSample Type：serum -
Oxidized high-density lipoprotein enhances endocrine disorders and ovarian damage in rats
IF: 5.310Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021)PMID：34346538Reactivity：RatSample Type：serum -
Icariin improves testicular dysfunction via enhancing proliferation and inhibiting mitochondria-dependent apoptosis pathway in high-fat diet and streptozotocin-induced diabetic rats
IF: 5.211Journal： Reproductive Biology and Endocrinology (2021)PMID：34753504Reactivity：RatSample Type：serum -
Pathological changes and oxidative stress of the HPG axis in hypothyroid rat
IF: 5.098 -
Evaluation of maternal high-fat diet and Quercetin-3-O-rutinoside treatment on the reproductive profile of diet na?ve male offspring
IF: 5.037 -
Antioxidant and cytoprotective effects of Nigella sativa L. seeds on the testis of monosodium glutamate challenged rats
IF: 4.380 -
Effects of prenatal chlorocholine chloride exposure on pubertal development and reproduction of male offspring in rats
IF: 4.374
实验操作视频
