Rat LH(Luteinizing Hormone) ELISA Kit
中文名称：大鼠促黄体生成激素(LH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 1.56-100 mIU/mL
- 灵敏度： 0.94 mIU/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠LH抗体包被于酶标板上，实验时样品（或标准品）中的大鼠LH会与包被抗体结合。后依次加入生物素化的抗大鼠LH抗体和辣根过氧化物酶标记的亲和素，抗大鼠LH抗体与结合在包被抗体上的大鼠LH结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LH浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LH的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 mIU/mL |
| 灵敏度 | 0.94 mIU/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠LH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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The gut-microbiota-testis axis mediated by the activation of the Nrf2 antioxidant pathway is related to prepuberal steroidogenesis disorders induced by di-(2-ethylhexyl) phthalate
IF: 3.056Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2020)PMID：32588312Reactivity：RatSample Type：Serum,Tissue homogenate -
Kolaviron suppresses dysfunctional reproductive axis associated with multi-walled carbon nanotubes exposure in male rats
IF: 3.056Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2020)PMID：32812151Reactivity：RatSample Type：Serum,Tissue homogenate -
Synergistic effect of curcumin and chitosan nanoparticles on nano-hydroxyapatite-induced reproductive toxicity in rats
IF: 3.056Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2020)PMID：33141380Reactivity：RatSample Type：Plasma -
Orlistat attenuates obesity-induced decline in steroidogenesis and spermatogenesis by up-regulating steroidogenic genes
IF: 2.860 -
Gestational Exposure to Synthetic Steroid Hormones Impaired Sperm Quantity and Quality in Wistar Rats
IF: 2.299Journal： International Journal of Endocrinology (2020)PMID：32148483Reactivity：RatSample Type：Serum -
Cilostazol protects against cyclophosphamide-induced ovarian toxicity in female rats: role of cAMP and HO-1
IF: 2.295 -
Spironolactone reversed hepato-ovarian triglyceride accumulation caused by letrozole-induced polycystic ovarian syndrome: tissue uric acid—a familiar foe
IF: 2.050Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2020)PMID：31925474Reactivity：RatSample Type：plasma -
Selenium attenuates diclofenac-induced testicular and epididymal toxicity in rats
IF: 1.951 -
Regulation of the hypothalamic GnRH–GnIH system by putrescine in adult female rats and GT1-7 neuronal cell line
IF: 1.917Journal： Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2020)DOI：10.1002/jez.2351PMID：32039555Reactivity：RatSample Type：serum -
Effects of prenatal and lactational bisphenol a and/or di(2-ethylhexyl) phthalate exposure on male reproductive system
IF: 1.916Journal： INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH (2020)PMID：32787440Reactivity：RatSample Type：Plasma
实验操作视频
