Rat LH(Luteinizing Hormone) ELISA Kit
中文名称：大鼠促黄体生成激素(LH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 1.56-100 mIU/mL
- 灵敏度： 0.94 mIU/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠LH抗体包被于酶标板上，实验时样品（或标准品）中的大鼠LH会与包被抗体结合。后依次加入生物素化的抗大鼠LH抗体和辣根过氧化物酶标记的亲和素，抗大鼠LH抗体与结合在包被抗体上的大鼠LH结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LH浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LH的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 mIU/mL |
| 灵敏度 | 0.94 mIU/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠LH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Duzhong Butiansu Prescription Improves Heat Stress-Induced Spermatogenic Dysfunction by Regulating Sperm Formation and Heat Stress Pathway
IF: 1.813Journal： Evidence-based Complementary and Alternative Medicine (2020)PMID：32184896Reactivity：RatSample Type：Serum -
Effects of 5-Hydroxymethylfurfural on Pubertal Development of Female Wistar Rats
IF: 1.803Journal： Journal of Clinical Research in Pediatric Endocrinology (2020)PMID：31475510Reactivity：RatSample Type：Serum -
Opposite effects of high- and low-dose di-(2-ethylhexyl) phthalate (DEHP) exposure on puberty onset, oestrous cycle regularity and hypothalamic kisspeptin expression in female rats
IF: 1.721Journal： REPRODUCTION FERTILITY AND DEVELOPMENT (2020)DOI：10.1071/RD19024PMID：32209209Reactivity：RatSample Type：serum -
Ferulic acid influences Nrf2 activation to restore testicular tissue from cadmium-induced oxidative challenge, inflammation, and apoptosis in rats
IF: 1.662Journal： JOURNAL OF FOOD BIOCHEMISTRY (2020)PMID：33047361Reactivity：RatSample Type：Serum,Tissue homogenate -
Selenium abates reproductive dysfunction via attenuation of biometal accumulation, oxido-inflammatory stress and caspase-3 activation in male rats exposed to arsenic
IF: 5.714 -
Impact of binary waterborne mixtures of nickel and zinc on hypothalamic-pituitary-testicular axis in rats
IF: 5.108 -
The Effect of Schisandra chinensis Baillon on Cross-Talk between Oxidative Stress, Endoplasmic Reticulum Stress, and Mitochondrial Signaling Pathway in Testes of Varicocele-Induced SD Rat
IF: 4.183Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019)PMID：31744253Reactivity：RatSample Type：serum -
Cadmium exposure during prenatal development causes testosterone disruption in multigeneration via SF-1 signaling in rats
IF: 3.775 -
Cholestasis-associated reproductive toxicity in male and female rats: The fundamental role of mitochondrial impairment and oxidative stress
IF: 3.499 -
Effects of chlorocholine chloride on pubertal development and reproductive functions in male rats
IF: 3.499
实验操作视频
