Rat LH(Luteinizing Hormone) ELISA Kit
中文名称：大鼠促黄体生成激素(LH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 1.56-100 mIU/mL
- 灵敏度： 0.94 mIU/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠LH抗体包被于酶标板上，实验时样品（或标准品）中的大鼠LH会与包被抗体结合。后依次加入生物素化的抗大鼠LH抗体和辣根过氧化物酶标记的亲和素，抗大鼠LH抗体与结合在包被抗体上的大鼠LH结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LH浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LH的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 mIU/mL |
| 灵敏度 | 0.94 mIU/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠LH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Effects of noopept on cognitive functions and pubertal process in rats with diabetes
IF: 3.448 -
Protective role of glutamine against cadmium-induced testicular dysfunction in Wistar rats: Involvement of G6PD activity
IF: 3.448 -
Cross-talk between ER stress and mitochondrial pathway mediated adriamycin-induced testicular toxicity and DA-9401 modulate adriamycin-induced apoptosis in Sprague–Dawley rats
IF: 3.439 -
Very low dose spironolactone protects experimentally-induced polycystic ovarian syndrome from insulin-resistant metabolic disturbances by suppressing elevated circulating testosterone
IF: 3.407 -
Attenuation potentials of royal jelly against hydroxyurea-induced infertility through inhibiting oxidation and release of pro-inflammatory cytokines in male rats
IF: 2.914Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2019)PMID：31127524Reactivity：RatSample Type：serum -
Cannabinoid-deficient Benin republic hemp (Cannabis sativa L.) improves semen parameters by reducing prolactin and enhancing anti-oxidant status
IF: 2.479Journal： BMC Complementary and Alternative Medicine (2019)PMID：31208410Reactivity：RatSample Type：plasma -
The ameliorative effect of monotropein, astragalin, and spiraeoside on oxidative stress, endoplasmic reticulum stress, and mitochondrial signaling pathway in varicocelized rats
IF: 2.479Journal： BMC Complementary and Alternative Medicine (2019)PMID：31771569Reactivity：RatSample Type：Serum -
Effects of N-nitro L-arginine methyl ester and α-tocopherol on testicular oxidative stress caused by exposure to cigarette smoke
IF: 1.840 -
Intracerebroventricular injection of histamine induces the hypothalamic-pituitary-gonadal axis activation in male rats
IF: 3.125 -
GnRH dysregulation in polycystic ovarian syndrome (PCOS) is a manifestation of an altered neurotransmitter profile
IF: 2.852Journal： Reproductive Biology and Endocrinology (2018)PMID：29642911Reactivity：RatSample Type：Serum
实验操作视频
