Rat LH(Luteinizing Hormone) ELISA Kit
中文名称：大鼠促黄体生成激素(LH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 1.56-100 mIU/mL
- 灵敏度： 0.94 mIU/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠LH抗体包被于酶标板上，实验时样品（或标准品）中的大鼠LH会与包被抗体结合。后依次加入生物素化的抗大鼠LH抗体和辣根过氧化物酶标记的亲和素，抗大鼠LH抗体与结合在包被抗体上的大鼠LH结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LH浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LH的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 mIU/mL |
| 灵敏度 | 0.94 mIU/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠LH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Mitochondria-targeted antioxidant therapy for an animal model of PCOS-IR
IF: 2.784Journal： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2018)PMID：30431108Reactivity：RatSample Type：Serum -
Expression of FKBP52 in the ovaries of PCOS rats
IF: 2.784Journal： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2018)PMID：30483787Reactivity：RatSample Type：Serum -
Virgin coconut oil extract mitigates testicular-induced toxicity of alcohol use in antiretroviral therapy
IF: 2.734 -
Research on Establishment of Abnormal Phlegmatic Syndrome with Premature Ovarian Failure Rat Model and Effects of Balgham Munziq Treatment
IF: 2.064Journal： Evidence-based Complementary and Alternative Medicine (2018)PMID：29770151Reactivity：RatSample Type：serum -
Testicular microanatomical and hormonal alterations following use of antiretroviral therapy in Sprague Dawley rats: Role of Naringenin
IF: 1.588 -
Melatonin prevents and ameliorates lead-induced gonadotoxicity through antioxidative and hormonal mechanisms
IF: 1.255 -
ANP promotes proliferation and inhibits apoptosis of ovarian granulosa cells by NPRA/PGRMC1/EGFR complex and improves ovary functions of PCOS rats
IF: 5.965 -
Protective Effects of Fluvastatin on Reproductive Function in Obese Male Rats Induced by High-Fat Diet through Enhanced Signaling of mTOR
IF: 5.104 -
Naringenin attenuates highly active antiretroviral therapy-induced sperm DNA fragmentations and testicular toxicity in Sprague-Dawley rats
IF: 2.427 -
Adjuvant potential of virgin coconut oil extract on antiretroviral therapy-induced testicular toxicity: An ultrastructural study
IF: 1.458
实验操作视频
