Rat MMP-13(Matrix Metalloproteinase 13) ELISA Kit
中文名称：大鼠基质金属蛋白酶13(MMP-13)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠MMP-13抗体包被于酶标板上，实验时样品（或标准品）中的大鼠MMP-13会与包被抗体结合。后依次加入生物素化的抗大鼠MMP-13抗体和辣根过氧化物酶标记的亲和素，抗大鼠MMP-13抗体与结合在包被抗体上的大鼠MMP-13结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，MMP-13浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中MMP-13的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠MMP-13，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Autophagy inhibition mediated by intrauterine miR-1912-3p/CTSD programming participated in the susceptibility to osteoarthritis induced by prenatal dexamethasone exposure in male adult offspring rats
IF: 4.800 -
Anti-Inflammatory Effects of Boric Acid in Treating Knee Osteoarthritis: Biochemical and Histopathological Evaluation in Rat Model
IF: 3.900 -
The natural product salicin alleviates osteoarthritis progression by binding to IRE1α and inhibiting endoplasmic reticulum stress through the IRE1α-IκBα-p65 signaling pathway
IF: 12.153 -
Protective effect of ethanolic extract of Echinacea purpurea contained nanoparticles on meniscal/ligamentous injury induced osteoarthritis in obese male rats
IF: 4.996 -
Improving the symptoms of post-traumatic osteoarthritis by α2-macroglobulin-rich serum
IF: 3.342 -
Seahorse Protein Hydrolysate Ameliorates Proinflammatory Mediators and Cartilage Degradation on Posttraumatic Osteoarthritis with an Obesity Rat Model
IF: 3.246 -
Boswellia serrata Extract, 5-Loxin?, Prevents Joint Pain and Cartilage Degeneration in a Rat Model of Osteoarthritis through Inhibition of Inflammatory Responses and Restoration of Matrix Homeostasis
IF: 2.650Journal： Evidence-based Complementary and Alternative Medicine (2022)Reactivity：RatSample Type：serum -
Inhibition effect of Caragana sinica root extracts on Osteoarthritis through MAPKs, NF-κB signaling pathway
IF: 3.738Journal： International Journal of Medical Sciences (2021)PMID：33456343Reactivity：RatSample Type：serum -
Evaluation of cytokines in protective effect of docosahexaenoic acid in experimental achilles tendinopathy rat model induced with type-1 collagenase
IF: 3.417 -
Investigation of the efficacy of daidzein in experimental knee osteoarthritis-induced with monosodium iodoacetate in rats
IF: 2.394
实验操作视频
