Rat MMP-9(Matrix Metalloproteinase 9) ELISA Kit
中文名称：大鼠基质金属蛋白酶9(MMP-9)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 7.81-500 ng/mL
- 灵敏度： 4.69 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠MMP-9抗体包被于酶标板上，实验时样品（或标准品）中的大鼠MMP-9会与包被抗体结合。后依次加入生物素化的抗大鼠MMP-9抗体和辣根过氧化物酶标记的亲和素，抗大鼠MMP-9抗体与结合在包被抗体上的大鼠MMP-9结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，MMP-9浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中MMP-9的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 ng/mL |
| 灵敏度 | 4.69 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠MMP-9，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Metabolites of gut microbiota fermenting Poria cocos polysaccharide alleviates chronic nonbacterial prostatitis in rats
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Protective Effect of Liposomal Epigallocatechin-Gallate in Experimental Gentamicin-Induced Hepatotoxicity
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Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an unmet medical need
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Huangbai liniment and berberine promoted wound healing in high-fat diet/Streptozotocin-induced diabetic rats
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Intragastric administration of Pien Tze Huang enhanced wound healing in diabetes by inhibiting inflammation and improving energy generation
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Neuroprotective Effects of a Cardioplegic Combination (Adenosine, Lidocaine, and Magnesium) in an Ischemic Stroke Model
IF: 5.682Journal： MOLECULAR NEUROBIOLOGY (2022)Reactivity：RatSample Type：blood -
Niacin mitigates blood–brain barrier tight junctional proteins dysregulation and cerebral inflammation in ketamine rat model of psychosis: Role of GPR109A receptor
IF: 5.201Journal： PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY (2022)PMID：35690118Reactivity：RatSample Type：Brain tissue -
Network pharmacology analysis uncovers the effect on apoptotic pathway by Bu-Fei formula for COPD treatment
IF: 5.195 -
Pharmacological and Toxicological Study of Coumarinolignoids from Cleome viscosa in Small Animals for the Management of Rheumatoid Arthritis
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实验操作视频
