Rat MMP-9(Matrix Metalloproteinase 9) ELISA Kit
中文名称:大鼠基质金属蛋白酶9(MMP-9)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 7.81-500 ng/mL
- 灵敏度: 4.69 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠MMP-9抗体包被于酶标板上,实验时样品(或标准品)中的大鼠MMP-9会与包被抗体结合。后依次加入生物素化的抗大鼠MMP-9抗体和辣根过氧化物酶标记的亲和素,抗大鼠MMP-9抗体与结合在包被抗体上的大鼠MMP-9结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,MMP-9浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中MMP-9的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 7.81-500 ng/mL |
灵敏度 | 4.69 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠MMP-9,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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Matrix metalloproteinase 9 a potential major player connecting atherosclerosis and osteoporosis in high fat diet fed rats
IF: 3.240 -
Exposure to Air Pollution Exacerbates Inflammation in Rats with Preexisting COPD
IF: 3.758Journal: MEDIATORS OF INFLAMMATION (2020)PMID:32454790Reactivity:RatSample Type:Tissue homogenate,Bronchoalveolar Lavage Fluid -
St. Thomas and del Nido cardioplegia are superior to Custodiol cardioplegia in a rat model of donor heart
IF: 1.084Journal: SCANDINAVIAN CARDIOVASCULAR JOURNAL (2020)PMID:33185130Reactivity:RatSample Type:Tissue homogenate -
Nisin reduces uterine inflammation in rats by modulating concentrations of pro- and anti-inflammatory cytokines
IF: 3.091Journal: AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2019)PMID:30681748Reactivity:RatSample Type:Serum -
The antiiflammatory effect of Ginkgo biloba in lipopolysaccharide-induced rat sepsis model
Journal: Journal of Laboratory Medicine (2018)Reactivity:RatSample Type:Serum -
Effect of chronic left ventricular unloading on myocardial remodeling: Multimodal assessment of two heterotopic heart transplantation techniques
IF: 5.611Journal: JOURNAL OF HEART AND LUNG TRANSPLANTATION (2014)PMID:25703962Reactivity:RatSample Type:Tissue homogenate