Rat MT(Melatonin) ELISA Kit
中文名称：大鼠褪黑素(MT)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用大鼠MT抗原包被于酶标板上，实验时样品或标准品中的大鼠MT与包被的大鼠MT竞争生物素标记的抗大鼠MT单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，大鼠MT浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中大鼠MT的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠MT，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Melatonin-coated nanofiber cell sheets promote periodontal regeneration through ROS scavenging and preservation of stemness
IF: 13.300 -
Exposure to four typical heavy metals induced telomere shortening of peripheral blood mononuclear cells in relevant with declined urinary aMT6s in rats
IF: 6.200Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2024)Reactivity：RatSample Type：Serum -
Phloretin Ameliorates against Bisphenol A Induced Polycystic Ovarian Syndrome, an Endocrine Disrupting Chemical, in Prepubertal Rats
IF: 0.500 -
Cardiac-targeted delivery of nuclear receptor RORα via ultrasound targeted microbubble destruction optimizes the benefits of regular dose of melatonin on sepsis-induced cardiomyopathy
IF: 11.300 -
Influence of different LED wavelengths on retinal melatonin levels - A rodent study
IF: 9.800Journal： SCIENCE OF THE TOTAL ENVIRONMENT (2023)PMID：37652369Reactivity：RatSample Type：retinal tissue -
Gut microbiota-derived melatonin from Puerariae Lobatae Radix-resistant starch supplementation attenuates ischemic stroke injury via a positive microbial co-occurrence pattern
IF: 9.300 -
Effects of Supplementation with the Standardized Extract of Saffron (affron?) on the Kynurenine Pathway and Melatonin Synthesis in Rats
IF: 7.000 -
Mechanism of GW117 antidepressant action: Melatonin receptor-mediated regulation of sleep rhythm
IF: 5.000 -
Subchronic Low-Dose Methylmercury Exposure Accelerated Cerebral Telomere Shortening in Relevant with Declined Urinary aMT6s Level in Rats
IF: 4.600 -
Subchronic Arsenite Exposure Induced Atrophy and Erythropoietin Sensitivity Reduction in Skeletal Muscle Were Relevant to Declined Serum Melatonin Levels in Middle-Aged Rats
IF: 4.600
实验操作视频
