Rat NFE2L2(Nuclear Factor, Erythroid Derived 2, Like 2) ELISA Kit
中文名称：大鼠红细胞衍生核因子2样蛋白2(NFE2L2)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠NFE2L2抗体包被于酶标板上，实验时样品（或标准品）中的大鼠NFE2L2会与包被抗体结合。后依次加入生物素化的抗大鼠NFE2L2抗体和辣根过氧化物酶标记的亲和素，抗大鼠NFE2L2抗体与结合在包被抗体上的大鼠NFE2L2结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，NFE2L2浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中NFE2L2的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠NFE2L2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Role of Anonychium africanum (Plantae, Fabaceae) in Metal Oxido-Inflammatory Response: Protection Evidence in Gonad of Male Albino Rat
IF: 6.000 -
Mangiferin mitigates methotrexate-induced liver injury and suppresses hepatic stellate cells activation in rats: Imperative role of Nrf2/NF-κB/NLRP3 signaling axis
IF: 4.800Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)Reactivity：RatSample Type：liver tissue -
Repurposing of niclosamide, an anthelmintic, by targeting ERK/MAPK signaling pathway?in the experimental paradigm of autism spectrum disorders
IF: 4.200Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2024)Reactivity：RatSample Type：brain tissue -
Omega-3 fatty acids abrogates oxido-inflammatory and mitochondrial dysfunction-associated apoptotic responses in testis of tamoxifen-treated rats
IF: 4.000Journal： Frontiers in Nutrition (2024)Reactivity：RatSample Type：Testicular tissue -
Exploring Astrocytes Involvement and Glutamate Induced Neuroinflammation in Chlorpyrifos-Induced Paradigm Of Autism Spectrum Disorders (ASD)
IF: 3.700Journal： NEUROCHEMICAL RESEARCH (2024)Reactivity：RatSample Type：brain tissue -
Testicular toxicity in cisplatin-treated Wistar rats is mitigated by Daflon and associated with modulation of Nrf2/HO-1 and TLR4/NF-kB signaling
IF: 3.600Journal： JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2024)Reactivity：RatSample Type：Testicular tissue -
Unlocking Synergistic Hepatoprotection: Dapagliflozin and Silymarin Combination Therapy Modulates Nuclear Erythroid 2-Related Factor 2/Heme Oxygenase-1 Pathway in Carbon Tetrachloride-Induced Hepatotoxicity in Wistar Rats
IF: 3.600 -
Zinc abates sodium benzoate -induced testicular dysfunction via upregulation of Nrf2/ HO-1/ Nf-κB signaling and androgen receptor gene
IF: 3.600Journal： JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2024)Reactivity：RatSample Type：Testicular tissue -
Orange Peel ethanolic extract and physical exercise prevent testicular toxicity in streptozocin and high fat diet-induced type 2 diabetes rats via Nrf2/NF-kB signaling: In silico and In vivo studies.
IF: 3.400Journal： Heliyon (2024)Reactivity：RatSample Type：Testicular tissue -
The impact of subdermal adipose derived stem cell injections and early excision on systemic oxidative stress and wound healing in rats with severe scald burns
IF: 3.200
实验操作视频
