Rat NGAL(NeutrophilGelatinase Associated Lipocalin) ELISA Kit
中文名称：大鼠中性粒细胞明胶酶相关脂质运载蛋白(NGAL)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.84 pg/mL
| 用途 | 试剂盒用于体外定量检测大鼠血清、血浆或其他生物体液中NGAL浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠NGAL抗体包被于酶标板上，实验时样品（或标准品）中的大鼠NGAL会与包被抗体结合。后依次加入生物素化的抗大鼠NGAL抗体和辣根过氧化物酶标记的亲和素，抗大鼠NGAL抗体与结合在包被抗体上的大鼠NGAL结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，NGAL浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中NGAL的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.84 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠NGAL，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Enhanced eNOS/nitric oxide production by nebivolol interferes with TGF-β1/Smad3 signaling and collagen I deposition in the kidney after prolonged tacrolimus administration
IF: 5.200 -
Circ_001653 alleviates sepsis associated-acute kidney injury by recruiting BUD13 to regulate KEAP1/NRF2/HO-1 signaling pathway
IF: 4.400Journal： Journal of Inflammation-London (2024)Reactivity：RatSample Type：urine tissue -
Renoprotective effect of esculetin against ischemic acute kidney injury-diabetic comorbidity
IF: 3.300 -
Exploring the impact of high-altitude de-acclimatization on renal function: The roles of oxidative and endoplasmic reticulum stress in rat models
IF: 3.100Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)PMID：38518722Reactivity：RatSample Type：urine -
Reno-Protective Effect of Fenofibrate and Febuxostat Against Vancomycin-Induced Acute Renal Injury in Rats: Targeting PPARγ/NF-κB/COX-II and AMPK/Nrf2/HO-1 Signaling Pathways
IF: 2.900Journal： IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2024)Reactivity：RatSample Type：serum -
Novel dysregulated long non-coding RNAs in the acute kidney injury-to-chronic kidney diseases transition unraveled by transcriptomic analysis
IF: 2.900Journal： Pharmacology Research & Perspectives (2024)Reactivity：RatSample Type：plasma -
Protective effect of β-sitosterol against high-fructose diet-induced oxidative stress, and hepatorenal derangements in growing female sprague-dawley rats
IF: 2.700Journal： Laboratory Animal Research (2024)Reactivity：RatSample Type：Plasma -
ER stress modulated Klotho restoration: A prophylactic therapeutic strategy against acute kidney injury-diabetes comorbidity
IF: 6.200Journal： BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2023)Reactivity：RatSample Type：plasma,urine samples -
Concomitant inhibition of TLR-4 and SGLT2 by phloretin and empagliflozin prevents diabetes-associated ischemic acute kidney injury
IF: 6.100 -
Salvianolic Acid A Improves Rat Kidney Injury by Regulating MAPKs and TGF-β1/Smads Signaling Pathways
IF: 4.600
实验操作视频
