Rat NOS2/iNOS(Nitric Oxide Synthase 2, Inducible) ELISA Kit
中文名称：大鼠诱导型一氧化氮合成酶(NOS2/iNOS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠NOS2/iNOS抗体包被于酶标板上，实验时样品（或标准品）中的大鼠NOS2/iNOS会与包被抗体结合。后依次加入生物素化的抗大鼠NOS2/iNOS抗体和辣根过氧化物酶标记的亲和素，抗大鼠NOS2/iNOS抗体与结合在包被抗体上的大鼠NOS2/iNOS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，NOS2/iNOS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中NOS2/iNOS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠NOS2/iNOS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Carthamus tinctorius L. protects cerebral ischemia/reperfusion injury via arachidonic acid/p53-mediated apoptosis axis
IF: 4.400Journal： Frontiers in Pharmacology (2024)Reactivity：RatSample Type：Serum -
Bioisosteric Replacement in the Search for Biologically Active Compounds: Design, Synthesis and Anti-Inflammatory Activity of Novel [1,2,4]triazino[2,3-c]quinazolines
IF: 4.300 -
Protective effect of deinoxanthin in sorafenib-induced nephrotoxicity in rats with the hepatocellular carcinoma model
IF: 3.100Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2024)Reactivity：RatSample Type：renal tissue -
Combined Bisoprolol and Trimetazidine ameliorate Arsenic trioxide -Induced Acute Myocardial Injury in Rats: Targeting PI3K/GSK-3β/Nrf2/HO-1 and NF-κB/iNOS Signaling Pathways, Inflammatory Mediators and Apoptosis
IF: 2.900Journal： IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2024)Reactivity：RatSample Type：Cardiac tissue -
Agomelatine-loaded nanostructured lipid carriers alleviate neuropathic pain in rats by Nrf2/HO-1 signalling pathway
IF: 2.400Journal： CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY (2024)Reactivity：RatSample Type：sciatic nerve tissue,spinal cord tissue -
Edaravone dexborneol regulates γ-aminobutyric acid transaminase in rats with acute intracerebral hemorrhage
IF: 2.000Journal： Journal of Stroke & Cerebrovascular Diseases (2024)Reactivity：RatSample Type：brain tissue -
Muscone Abates 1,2-Dimethylhydrazine-Induced Colon Cancer in Rats and HCT-116 Cells Proliferation via Modulation of TNF-α, COX-2, NF-kB, and Phase I Xenobiotic Enzymes
IF: 1.400Journal： Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy (2024)Reactivity：RatSample Type：serum -
Artificially reprogrammed stem cells deliver transcytosable nanocomplexes for improved spinal cord repair
IF: 10.800 -
Restoring glomerular filtration rate by sulforaphane modulates ERK1/2/JNK/p38MAPK, IRF3/iNOS, Nrf2/HO-1 signaling pathways against folic acid-induced acute renal injury in rats
IF: 5.600Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2023)PMID：37567014Reactivity：RatSample Type：Renal tissue -
Shiwei Qingwen decoction regulates TLR4/NF-κB signaling pathway and NLRP3 inflammasome to reduce inflammatory response in lipopolysaccharide-induced acute lung injury
IF: 5.400Journal： JOURNAL OF ETHNOPHARMACOLOGY (2023)PMID：37164255Reactivity：RatSample Type：Bronchoalveolar lavage fluid(BALF)
实验操作视频
