Rat NSE(Neuron Specific Enolase) ELISA Kit
中文名称：大鼠神经特异性烯醇化酶(NSE)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠NSE抗体包被于酶标板上，实验时样品（或标准品）中的大鼠NSE会与包被抗体结合。后依次加入生物素化的抗大鼠NSE抗体和辣根过氧化物酶标记的亲和素，抗大鼠NSE抗体与结合在包被抗体上的大鼠NSE结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，NSE浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中NSE的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠NSE，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Acute hyperglycemia exacerbates neuroinflammation and cognitive impairment in sepsis-associated encephalopathy by mediating the ChREBP/HIF-1α pathway
IF: 2.800Journal： EUROPEAN JOURNAL OF MEDICAL RESEARCH (2024)Reactivity：RatSample Type：Serum -
Ac2-26 activated the AKT1/GSK3β pathway to reduce cerebral neurons pyroptosis and improve cerebral function in rats after cardiopulmonary bypass
IF: 2.000Journal： BMC Cardiovascular Disorders (2024)Reactivity：RatSample Type：serum -
A Nanotherapy of Octanoic Acid Ameliorates Cardiac Arrest/Cardiopulmonary Resuscitation-Induced Brain Injury via RVG29- and Neutrophil Membrane-Mediated Injury Relay Targeting
IF: 17.100 -
Optimal inhaled oxygen and carbon dioxide concentrations for post-cardiac arrest cerebral reoxygenation and neurological recovery
IF: 5.800Journal： iScience (2023)Reactivity：RatSample Type：plasma,brain,heart tissue -
Ferroptosis contributes to hemolytic hyperbilirubinemia?induced brain damage in?vivo and in vitro
IF: 3.400 -
Inhaled Carbon Dioxide Improves Neurological Outcomes by Downregulating Hippocampal Autophagy and Apoptosis in an Asphyxia‐Induced Cardiac Arrest and Resuscitation Rat Model
IF: 6.106Journal： Journal of the American Heart Association (2022)PMID：36314493Reactivity：RatSample Type：blood -
Neuroprotective Effects of a Cardioplegic Combination (Adenosine, Lidocaine, and Magnesium) in an Ischemic Stroke Model
IF: 5.682Journal： MOLECULAR NEUROBIOLOGY (2022)Reactivity：RatSample Type：blood -
Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway
IF: 3.889 -
Protective effect of S-allyl cysteine against cerebral ischemia/reperfusion injury in experimental rats
IF: 0.533Journal： TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH (2021)Reactivity：RatSample Type：brain -
Neuroprotective Effect of the Inhibitor Salubrinal after Cardiac Arrest in a Rodent Model
IF: 5.076Journal： Oxidative Medicine and Cellular Longevity (2020)PMID：32064028Reactivity：RatSample Type：Serum
实验操作视频
