Rat PAI1(PlasminogenActivator Inhibitor 1) ELISA Kit
中文名称：大鼠纤溶酶原激活物抑制因子1(PAI1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 78.13-5000 pg/mL
- 灵敏度： 32.15 pg/mL
| 用途 | 试剂盒用于体外定量检测大鼠血清、血浆或其他生物体液中PAI1浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠PAI1抗体包被于酶标板上，实验时样品（或标准品）中的大鼠PAI1会与包被抗体结合。后依次加入生物素化的抗大鼠PAI1抗体和辣根过氧化物酶标记的亲和素，抗大鼠PAI1抗体与结合在包被抗体上的大鼠PAI1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，PAI1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中PAI1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 78.13-5000 pg/mL |
| 灵敏度 | 32.15 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠PAI1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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High salt diet accelerates skin aging in wistar rats: an 8-week investigation of cell cycle inhibitors, SASP markers, and oxidative stress
IF: 4.300Journal： Frontiers in Bioengineering and Biotechnology (2024)Reactivity：RatSample Type：skin tissue -
The effects of resistance training on cardiovascular factors and anti-inflammation in diabetic rats
IF: 3.400 -
Impact of L-Arginine on diabetes-induced neuropathy and myopathy: Roles of PAI-1, Irisin, oxidative stress, NF-κβ, autophagy and microRNA-29a
IF: 2.600 -
Patchouli alcohol against renal fibrosis of spontaneously hypertensive rats via Ras/Raf-1/ERK1/2 signalling pathway
IF: 3.300Journal： JOURNAL OF PHARMACY AND PHARMACOLOGY (2023)PMID：37177974Reactivity：RatSample Type：plasma,renal -
Allopurinol and valproic acid improve cardiac triglyceride and Na+-K+-ATPase activity independent of circulating aldosterone in female rats with glucose intolerance
IF: 2.575Journal： ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2020)PMID：32447998Reactivity：RatSample Type：plasma -
Chronic intermittent hypoxia vs chronic continuous hypoxia: Effects on vascular endothelial function and myocardial contractility
IF: 1.741Journal： CLINICAL HEMORHEOLOGY AND MICROCIRCULATION (2020)PMID：31683472Reactivity：RatSample Type：serum -
Combined but not single treatment with ethinylestradiol/levonorgestrel and spironolactone reduces plasminogen activator inhibitor-1 in insulin-resistant ovariectomised rats
IF: 1.356Journal： JOURNAL OF THE RENIN-ANGIOTENSIN-ALDOSTERONE SYSTEM (2019)PMID：31856649Reactivity：RatSample Type：Plasma -
Antioxidant and anti-inflammatory activities of alpha lipoic acid protect against indomethacin-induced gastric ulcer in rats
IF: 3.457Journal： BIOMEDICINE & PHARMACOTHERAPY (2018)PMID：29486337Reactivity：RatSample Type：Tissue homogenate -
Amitriptyline attenuates bleomycin-induced pulmonary fibrosis: modulation of the expression of NF-κβ, iNOS, and Nrf2
IF: 2.238Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2018)PMID：30474696Reactivity：RatSample Type：Tissue homogenate -
Ameliorative effect of nicotine exposure on insulin resistance is accompanied by decreased cardiac glycogen synthase kinase-3 and plasminogen activator inhibitor-1 during oral oestrogen-progestin therapy
IF: 1.220Journal： ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2017)PMID：28868937Reactivity：RatSample Type：Plasma,Tissue homogenate
实验操作视频
