Rat PRL(Prolactin) ELISA Kit
中文名称：大鼠催乳素(PRL)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 3.13-200 ng/mL
- 灵敏度： 1.88 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠PRL抗体包被于酶标板上，实验时样品（或标准品）中的大鼠PRL会与包被抗体结合。后依次加入生物素化的抗大鼠PRL抗体和辣根过氧化物酶标记的亲和素，抗大鼠PRL抗体与结合在包被抗体上的大鼠PRL结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，PRL浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中PRL的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 3.13-200 ng/mL |
| 灵敏度 | 1.88 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠PRL，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Androgen Receptor Mediates Dopamine Agonist Resistance by Regulating Intracellular reactive oxygen species (ROS) in Prolactin-secreting Pituitary Adenoma
IF: 5.900Journal： ANTIOXIDANTS & REDOX SIGNALING (2024)Reactivity：Rat,MouseSample Type：serum -
The Activation of p300 Enhances the Sensitivity of Pituitary Adenomas to Dopamine Agonist Treatment by Regulating the Transcription of DRD2
IF: 4.900Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity：Rat -
Exogenous taurine administration abates reproductive dysfunction in male rats exposed to silver nanoparticles
IF: 4.500 -
Attenuation of doxorubicin-induced hypothalamic-pituitary-testicular axis dysfunction by diphenyl diselenide involves suppression of hormonal deficits, oxido-inflammatory stress and caspase 3 activity in rats
IF: 3.500Journal： JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2023)PMID：37379681Reactivity：RatSample Type：serum -
Zinc and selenium attenuate quaternary heavy metal mixture-induced testicular damage via amplification of the antioxidant system, reduction in metal accumulation, inflammatory and apoptotic biomarkers
IF: 2.300 -
PBK/TOPK Inhibitor Suppresses the Progression of Prolactinomas
IF: 6.055 -
Beneficial effects of irisin in experimental paroxetine-induced hyperprolactinemia
IF: 3.925 -
The elusive role of prolactin in the sociality of the naked mole-rat
IF: 3.492 -
Postpartum depression in rats causes poor maternal care and neurochemical alterations on dams and long-lasting impairment in sociability on the offspring
IF: 3.352 -
Behavioral and hormonal effects of prenatal and maternal separation stresses in postpartum rats
IF: 1.708
实验操作视频
