Rat REN (Renin) ELISA Kit
中文名称:大鼠肾素(REN)酶联免疫吸附测定试剂盒
- 货号:E-EL-R3075
- 文献(31)
Price:
- 反应性: Rat
- 检测范围: 1.56-100 pg/mL
- 灵敏度: 0.94 pg/mL
用途 | 试剂盒用于体外定量检测大鼠血清、血浆或其他生物体液中REN浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠REN抗体包被于酶标板上,实验时样品(或标准品)中的大鼠REN会与包被抗体结合。后依次加入生物素化的抗大鼠REN体和辣根过氧化物酶标记的亲和素,抗大鼠REN抗体与结合在包被抗体上的大鼠REN结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,REN浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中REN的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 1.56-100 pg/mL |
灵敏度 | 0.94 pg/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠REN,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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Valsartan chronotherapy reverts the non-dipper pattern and improves blood pressure control through mediation of circadian rhythms of the renin-angiotensin system in spontaneous hypertension rats
IF: 2.562 -
Antihypertensive effects of the hydro-ethanol extract of Senecio serratuloides DC in rats
IF: 2.479Journal: BMC Complementary and Alternative Medicine (2019)PMID:30819180Reactivity:MouseSample Type:Serum -
The effect of eplerenone on the renin-angiotensin-aldosterone system of rats with thyroid dysfunction
IF: 2.390 -
Aliskiren, Fosinopril, and Their Outcome on Renin-Angiotensin-Aldosterone System (RAAS) in Rats with Thyroid Dysfunction
IF: 2.287Journal: International Journal of Endocrinology (2019)PMID:31396276Reactivity:RatSample Type:serum -
Blood pressure lowering effect of Ficus deltoidea var kunstleri in spontaneously hypertensive rats: possible involvement of renin–angiotensin–aldosterone system, endothelial function and anti-oxidant system
IF: 2.107 -
Standardized ethanol-water extract of Ficus deltoidea Angustifolia reduces blood pressure in spontaneously hypertensive rats
IF: 1.522Journal: CLINICAL AND EXPERIMENTAL HYPERTENSION (2019)PMID:30648895Reactivity:RatSample Type:Serum -
Effects and mechanism of total phenols of Magnolia officinalis combined with Maijunan Tablets on blood pressure of spontaneous hypertensive rats
Journal: Chinese Herbal Medicines (2019)PMID:36121411Reactivity:RatSample Type:Plasma,Serum,Tissue homogenate -
Antihypertensive Effect of Ethanolic Extract from Acanthopanax sessiliflorus Fruits and Quality Control of Active Compounds
IF: 4.936Journal: Oxidative Medicine and Cellular Longevity (2018)PMID:29849899Reactivity:RatSample Type:serum -
Calcimimetic R568 reduced the blood pressure and improved aortic remodeling in spontaneously hypertensive rats by inhibiting local renin?angiotensin system activity
IF: 1.410Journal: Experimental and Therapeutic Medicine (2018)PMID:30402152Reactivity:RatSample Type:Plasma,Tissue homogenate -
Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS
IF: 2.861