Rat SDC1(Syndecan 1) ELISA Kit
中文名称：大鼠多配体聚糖1(SDC1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠SDC1抗体包被于酶标板上，实验时样品（或标准品）中的大鼠SDC1会与包被抗体结合。后依次加入生物素化的抗大鼠SDC1抗体和辣根过氧化物酶标记的亲和素，抗大鼠SDC1抗体与结合在包被抗体上的大鼠SDC1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，SDC1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中SDC1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠SDC1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Protective Effect of Oxygen and Isoflurane in Rodent Model of Intestinal Ischemia-Reperfusion Injury
IF: 5.600Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)PMID：36768910Reactivity：RatSample Type：Plasma -
Intravenous Polyethylene Glycol Alleviates Intestinal Ischemia-Reperfusion Injury in a Rodent Model
IF: 5.600Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)PMID：37445954Reactivity：RatSample Type：plasma -
The Effects of Heparan Sulfate Infusion on Endothelial and Organ Injury in a Rat Pneumosepsis Model
IF: 3.900 -
The effects of resuscitation with different plasma products on endothelial permeability and organ injury in a rat pneumosepsis model
IF: 3.500Journal： Intensive Care Medicine Experimental (2023)PMID：37728777Reactivity：RatSample Type：liver,kidney -
Albumin protects the ultrastructure of the endothelial glycocalyx of coronary arteries in myocardial ischemia-reperfusion injury in?vivo
IF: 3.100Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2023)PMID：37172449Reactivity：RatSample Type：Plasma -
The Role of IGL-2 Preservation Solution on Rat Livers during SCS and HOPE
IF: 6.208Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)PMID：36293465Reactivity：RatSample Type：livers -
LncRNA H19 deficiency protects against the structural damage of glomerular endothelium in diabetic nephropathy via Akt/eNOS pathway
IF: 3.188Journal： ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2022)Reactivity：RatSample Type：serum -
Fluid Resuscitation Aggravates the Cellular Injury in a Hemorrhagic Shock Model
-
Bosutinib reduces endothelial permeability and organ failure in a rat polytrauma transfusion model
IF: 9.166 -
Plasma and rhADAMTS13 reduce trauma-induced organ failure by restoring the ADAMTS13-VWF axis
IF: 6.799
实验操作视频
