Rat SIRT1(Sirtuin 1) ELISA Kit
中文名称：大鼠沉默调节蛋白1(SIRT1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠SIRT1抗体包被于酶标板上，实验时样品（或标准品）中的大鼠SIRT1会与包被抗体结合。后依次加入生物素化的抗大鼠SIRT1抗体和辣根过氧化物酶标记的亲和素，抗大鼠SIRT1抗体与结合在包被抗体上的大鼠SIRT1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，SIRT1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中SIRT1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠SIRT1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Potential neuroprotective and autophagy-enhancing effects of alogliptin on lithium/pilocarpine-induced seizures in rats: Targeting the AMPK/SIRT1/Nrf2 axis
IF: 5.200Journal： LIFE SCIENCES (2024)Reactivity：RatSample Type：hippocampal tissue -
Astragalus polysaccharides attenuate rat aortic endothelial senescence via regulation of the SIRT-1/p53 signaling pathway
IF: 3.900Journal： BMC Complementary Medicine and Therapies (2024)PMID：38331805Reactivity：RatSample Type：serum -
Activation of SIRT1/Nrf2/HO-1 and Beclin-1/AMPK/mTOR autophagy pathways by eprosartan ameliorates testicular dysfunction induced by testicular torsion in rats
IF: 3.800 -
Mahanimbine Attenuates Ovariectomy-Induced Osteoporosis in Rats by Regulating Bone Turnover Markers and SIRT1-NF-κB Pathway
IF: 1.400Journal： Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy (2024)Reactivity：RatSample Type：serum -
Niacin modulates depressive-like behavior in experimental colitis through GPR109A-dependent mechanisms
IF: 6.100 -
The effects of triblock copolymer nano-vesicles loaded with propolis on heart failure model of rats
IF: 6.000Journal： Arabian Journal of Chemistry (2023)Reactivity：RatSample Type：plasma -
Acetyl-11-Keto-Beta Boswellic Acid(AKBA) modulates CSTC-pathway by activating SIRT-1/Nrf2-HO-1 signalling in experimental rat model of obsessive-compulsive disorder: Evidenced by CSF, blood plasma and histopathological alterations
IF: 3.400Journal： NEUROTOXICOLOGY (2023)PMID：37549874Reactivity：RatSample Type：brain,plasma,cerebrospinal fluid (CSF) -
Effect of Oleanolic acid administration on hepatic AMPK, SIRT-1, IL-6 and NF-κB levels in experimental diabetes
IF: 2.800Journal： Journal of Diabetes and Metabolic Disorders (2023)PMID：37255809Reactivity：RatSample Type：Liver tissue -
Amentoflavone Mitigates Cyclophosphamide-Induced Pulmonary Toxicity: Involvement of -SIRT-1/Nrf2/Keap1 Axis, JAK-2/STAT-3 Signaling, and Apoptosis
IF: 2.600 -
The Effects of Subtoxic Dose of Acetaminophen Combined With Exercise on the Liver of Rats
IF: 2.100
实验操作视频
