Rat SIRT1(Sirtuin 1) ELISA Kit
中文名称:大鼠沉默调节蛋白1(SIRT1)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠SIRT1抗体包被于酶标板上,实验时样品(或标准品)中的大鼠SIRT1会与包被抗体结合。后依次加入生物素化的抗大鼠SIRT1抗体和辣根过氧化物酶标记的亲和素,抗大鼠SIRT1抗体与结合在包被抗体上的大鼠SIRT1结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,SIRT1浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中SIRT1的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠SIRT1,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
The effects of Spirulina platensis on behavior in adolescent rats fed a high-fat diet
IF: 1.800Journal: INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE (2023)PMID:37315948Reactivity:RatSample Type:brain tissue -
Resveratrol attenuates behavioural impairment associated with learning and memory in rats with diabetes induced by a high-fat diet and streptozotocin
IF: 9.473Journal: BRITISH JOURNAL OF PHARMACOLOGY (2022)PMID:35710260Reactivity:RatSample Type:plasma, brain -
Hepatoprotective and cognitive-enhancing effects of hesperidin against thioacetamide-induced hepatic encephalopathy in rats
IF: 6.780 -
Agomelatine ameliorates cadmium-induced toxicity through the modification of HMGB-1/TLR-4/NFκB pathway
IF: 4.460Journal: TOXICOLOGY AND APPLIED PHARMACOLOGY (2022)PMID:36356678Reactivity:RatSample Type:cardiac tissue,testicular tissue -
Berberine ameliorates doxorubicin-induced cognitive impairment (chemobrain) in rats
IF: 5.037