Rat VIP(Vasoactive Intestinal Peptide) ELISA Kit
中文名称：大鼠血管活性肠肽(VIP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用大鼠VIP抗原包被于酶标板上，实验时样品或标准品中的大鼠VIP与包被的大鼠VIP竞争生物素标记的抗大鼠VIP单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，大鼠VIP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中大鼠VIP的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠VIP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Effects of Bifidobacterium and rosuvastatin on metabolic-associated fatty liver disease via the gut–liver axis
IF: 3.900Journal： Lipids in Health and Disease (2024)Reactivity：RatSample Type：serum -
Stool-softening effect and action mechanism of free anthraquinones extracted from Rheum palmatum L. on water deficit-induced constipation in rats
IF: 5.400 -
Evaluation of the effectiveness and mechanism of action of the Chang-Kang-Fang formula combined with bifid triple viable capsules on diarrhea-predominant irritable bowel syndrome
IF: 5.200 -
Combination of pseudoephedrine and emodin ameliorates LPS-induced acute lung injury by regulating macrophage M1/M2 polarization through the VIP/cAMP/PKA pathway
IF: 4.546 -
Extracts of Poria cocos improve functional dyspepsia via regulating brain-gut peptides, immunity and repairing of gastrointestinal mucosa
IF: 5.340 -
Atractylodes oil alleviates diarrhea-predominant irritable bowel syndrome by regulating intestinal inflammation and intestinal barrier via SCF/c-kit and MLCK/MLC2 pathways
IF: 4.360Journal： JOURNAL OF ETHNOPHARMACOLOGY (2021)PMID：33592255Reactivity：RatSample Type：Serum,Tissue homogenate(colon) -
Laxative effect and mechanism of Tiantian Capsule on loperamide-induced constipation in rats
IF: 3.690 -
Cervical sympathectomy modulates the neurogenic inflammatory neuropeptides following experimental subarachnoid hemorrhage in rats
IF: 2.929 -
Modulatory effects of neuropeptides on pentylenetetrazol-induced epileptic seizures and neuroinflammation in rats
IF: 0.801Journal： Revista da Associacao Medica Brasileira (2019)PMID：31618336Reactivity：RatSample Type：Plasma
实验操作视频
