Rat VWF(Von Willebrand Factor) ELISA Kit
中文名称：大鼠血管性血友病因子(VWF)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠VWF抗体包被于酶标板上，实验时样品（或标准品）中的大鼠VWF会与包被抗体结合。后依次加入生物素化的抗大鼠VWF抗体和辣根过氧化物酶标记的亲和素，抗大鼠VWF抗体与结合在包被抗体上的大鼠VWF结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，VWF浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中VWF的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠VWF，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
The cardioprotective effects of Fruitflow? against Doxorubicin-induced toxicity in rat cardiomyoblast cells H9c2 (2?1) and high-fat diet-induced dyslipidemia and pathological alteration in cardiac tissue of Wistar Albino rats
IF: 6.900Journal： BIOMEDICINE & PHARMACOTHERAPY (2024)Reactivity：RatSample Type：heart tissue -
Revisiting Virchow’s triad: exploring the cellular and molecular alterations in cerebral venous congestion
IF: 6.100Journal： Cell and Bioscience (2024)Reactivity：RatSample Type：cerebral venous sinuse tissue -
Zolmitriptan niosomal transdermal patches: combating migraine via epigenetic and endocannabinoid pathways and reversal of migraine hypercoagulability
IF: 5.700Journal： Drug Delivery and Translational Research (2024)Reactivity：RatSample Type：brain tissue -
Edible bird’s nest attenuates procoagulation effects of high-fat diet in rats
IF: 4.800 -
The Effects of Heparan Sulfate Infusion on Endothelial and Organ Injury in a Rat Pneumosepsis Model
IF: 3.900 -
Transfusion of female blood in a rat model is associated with red blood cells entrapment in organs
IF: 3.700 -
The effects of resuscitation with different plasma products on endothelial permeability and organ injury in a rat pneumosepsis model
IF: 3.500Journal： Intensive Care Medicine Experimental (2023)PMID：37728777Reactivity：RatSample Type：liver,kidney -
Extracellular Vesicles as an Index for Endothelial Injury and Cardiac Dysfunction in a Rodent Model of GDM
IF: 6.208Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)PMID：35563365Reactivity：RatSample Type：plasma -
Validation of the relationship between coagulopathy and localization of hydroxyethyl starch on the vascular endothelium in a rat hemodilution model
IF: 4.380 -
Salvianolic acid A prevented cerebrovascular endothelial injury caused by acute ischemic stroke through inhibiting the Src signaling pathway
IF: 5.064
实验操作视频
