Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Red)
中文名称:活性氧(ROS)荧光法测试盒(红色)

Price:
Size:
96 T
- 样本类型: 细胞
- 检测仪器及波长: 荧光酶标仪、流式细胞仪或荧光显微镜
- 研究领域: 氧化应激;铁死亡;能量代谢
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根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
活性氧是指机体内或者自然环境中由氧组成, 含氧并且性质活泼的物质的总称:主要有一种激发态的氧分子,即一重态氧分子或称单线态氧分子,3种含氧的自由基,即超氧阴离子自由基、羟自由基和氢过氧自由基,2种过氧化物, 即过氧化氢和过氧化脂质以及一种含氮的氧化物等。二氢乙锭可以穿过细胞膜进入细胞内与DNA或RNA结合,形成氢乙锭产生红色荧光。二氢乙锭在细胞内主要被超氧阴离子型活性氧氧化。
二氢乙锭本身为蓝色荧光,最大激发波长为370 nm,最大发射波长为 420 nm,脱氢后与RNA或DNA结合产生红色荧光,激发波长为300 nm或518 nm,发射波长为610 nm。 |
指标简称 | ROS |
检测时间 | 1 h 30 min |
检测仪器及波长 | 荧光酶标仪、流式细胞仪或荧光显微镜 |
保存条件 | 该产品可在-20°C避光条件下保存6个月。 |
有效期 | 6个月 |
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