Recombinant Human ACE2 Protein (His Tag)
Price:
- 表达系统： HEK293 Cells
- 蛋白编码： Q9BYF1
- 应用性： BLI
| 别称 |
ACE-Related Carboxypeptidase;ACE2;ACEH;Angiotensin-Converting Enzyme 2;Angiotensin-Converting Enzyme Homolog;Metalloprotease MPROT15
|
| 表达系统 |
HEK293 Cells
|
| 序列 |
Gln18-Ser740
|
| 蛋白编码 |
Q9BYF1
|
| 种属 |
Human
|
| 计算分子量 |
84.6 kDa
|
| 表观分子量 |
103 kDa
|
| 标签 |
C-His
|
| 生物活性 |
Loaded 2019-nCoV S Protein RBD-mFc on AMC Biosensor, can bind Human ACE-2-His with an affinity constant of 2.06 nM as determined in BLI assay.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
|
| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 300mM NaCl, 1mM ZnCl2, 10% Glycerol, pH 7.4.
|
| 复溶方法 |
Not Applicable
|
| 背景 |
Angiotensin-Converting Enzyme 2 (ACE-2) is an integral membrane protein and a zinc metalloprotease of the ACE family, the ACE family includes somatic and germinal ACE. ACE-2 cleaves angiotensins I and II as a carboxypeptidase, ACE-2 converts angiotensin I to angiotensin 1-9, and angiotensin II to angiotensin 1-7. ACE-2 is also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. ACE-2 can be high expressed in testis, kidney and heart, in colon, small intestine and ovary at moderate levels. Captopril and lisinopril as the classical ACE inhibitor don’t inhibit ACE-2 activity. ACE-2 may play an important role in regulating the heart function.
|
-
Targeting the Neuropilin-1 receptor with Ovatodiolide and progress in using periodontal ligament organoids for COVID-19 research and therapy
IF: 5.200Journal： LIFE SCIENCES (2024) -
Incorporation of Thiol-Modified and Carboxyl-MoS2 Films as Support Layer in Surface Plasmon Resonance Sensing Platform for SARS-CoV-2 Spike Protein Detection
IF: 4.300Journal： IEEE SENSORS JOURNAL (2024) -
Establishment of in-house assay for screening of anti-SARS-CoV-2 protein inhibitors
IF: 3.500 -
Design of Three Residues Peptides against SARS-CoV-2 Infection
IF: 5.818
Q1:重组蛋白有活性吗？可以做活性检测吗？
活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。
Q2:重组蛋白是否测序验证过？
在售重组蛋白默认不做测序分析/验证。
Q3:蛋白的运输条件是什么？
我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。
Q4:蛋白是通过什么方式纯化的？
一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。
Q5:蛋白可以用于细胞培养/注射小鼠吗？
内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。
Q6:蛋白发货形式可以是冻干粉吗？可以发液体吗？
默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。
Q7:蛋白Buffer里是否含有denaturants（变性剂）？
我们在售重组蛋白一般不含变性剂，具体见说明书的formulation信息。常见变性剂有：尿素和盐酸胍。
Q8:无标签蛋白纯化方式？
蛋白的纯化方式一般通过标签进行亲和纯化，无标签的蛋白是在纯化后进行了标签切除。
Q9:AF蛋白可以提供吗？
在售的重组蛋白都属于Animal component free protein。在售重组蛋白里不含任何直接来源与动物和人的制品：比如血清，血浆，全血，BSA，OVA等。
实验操作视频
新品推荐
Recombinant Mouse Ngfb Protein(His Tag)
Recombinant Mouse IL-18 Protein(Halo Tag)
Recombinant Mouse IL-11 Protein(Sumo Tag)
Recombinant Human VIM Protein(Halo Tag)
Recombinant Rat IL-2 Protein(His Tag)
Recombinant Human GCLC Protein(Trx Tag)
Recombinant Rat IL-17A Protein(hIgG1 Fc Tag)
Recombinant Human IL-17A Protein(Halo Tag)
联系我们
