Recombinant Human ACSL4 Protein(Sumo Tag)-Elabscience伊莱瑞特【官网】

Recombinant Human ACSL4 Protein(Sumo Tag)

一键复制产品信息

货号：PDEH100661
说明书

说明书

Recombinant Human ACSL4 Protein(Sumo Tag)

Price: ¥3600.00 ¥1200.00

Size:

100 μg 20 μg

表达系统： E.coli
蛋白编码： O60488

别称	ACS;ACSL;FACL;LACS;MRX;ACSL4;ACS4;FACL4;LACS4;MRX63;MRX68;ACS 4;ACSL 4;acyl CoA synthetase 4;Acyl CoA synthetase long chain family member 4;FACL 4;Fatty acid Coenzyme A ligase;fatty acid Coenzyme A ligase long-chain 4;LACS 4;Lignoceroyl CoA synthase;Long chain 4;long chain acyl CoA synthetase 4;long chain fatty acid CoA ligase 4;long chain fatty acid Coenzyme A ligase 4;Long-chain acyl-CoA synthetase 4;Long-chain-fatty-acid--CoA ligase 4
表达系统	E.coli
序列	Leu184-Tyr277
蛋白编码	O60488
种属	Human
计算分子量	26.3 kDa
表观分子量	30 kDa
标签	N-Sumo
生物活性	Not validated for activity
纯度	> 90% as determined by reducing SDS-PAGE.
内毒素	< 10 EU/mg of the protein as determined by the LAL method
保存条件	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输条件	This product is provided as lyophilized powder which is shipped with ice packs.
制剂	Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
复溶方法	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
背景	Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.Defects in ACSL4 are the cause of mental retardation X-linked type 63 (MRX63) [MIM:300387]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs. Defects in ACSL4 are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR) [MIM:300194]. A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis.

SDS-PAGE analysis of Human ACSL4 proteins, 2μg/lane of Recombinant Human ACSL4 proteins was resolved with SDS-PAGE under reducing conditions, showing bands at 30 KD

Q1:重组蛋白有活性吗？可以做活性检测吗？

活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。

Q2:重组蛋白是否测序验证过？

在售重组蛋白默认不做测序分析/验证。

Q3:蛋白的运输条件是什么？

我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。

Q4:蛋白是通过什么方式纯化的？

一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。

Q5:蛋白可以用于细胞培养/注射小鼠吗？

内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。

Q6:蛋白发货形式可以是冻干粉吗？可以发液体吗？

默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。