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Recombinant Human ACVRL1 Protein(His Tag)

Recombinant Human ACVRL1 Protein(His Tag)
  • Recombinant Human ACVRL1 Protein(His Tag)
  • Recombinant Human ACVRL1 Protein(His Tag)

Price: ¥3600.00 ¥1200.00

Size:
100 μg 20 μg
  • 表达系统: Mammalian
  • 蛋白编码: P37023
别称
Activin A receptor;Activin A receptor type II like 1;Activin A receptor;type II like kinase 1;Activin receptor like kinase 1;Activin receptor-like kinase 1;ACVL1;ACVRL1;ACVRLK1;ALK-1;ALK1;HHT;HHT2;ORW2;Osler Rendu Weber syndrome 2;Serine/threonine protein kinase receptor R3;Serine/threonine-protein kinase receptor R3;SKR3;TGF B superfamily receptor type I;TGF-B superfamily receptor type I;TSR-I;TSR1
表达系统
Mammalian
序列
Asp22-Gln118
蛋白编码
P37023
种属
Human
计算分子量
10.5 kDa
表观分子量
25-30 kDa
标签
C-His
生物活性
Not validated for activity
纯度
> 90% as determined by reducing SDS-PAGE.
内毒素
< 1.0 EU/mg of the protein as determined by the LAL method
保存条件
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输条件
This product is provided as lyophilized powder which is shipped with ice packs.
制剂
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
复溶方法
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
背景
This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with an other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine-and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with an other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with an hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2.
SDS-PAGE analysis of Human ACVRL1 proteins, 2 μg/lane of Recombinant Human ACVRL1 proteins was resolved with an SDS-PAGE under reducing conditions, showing bands at 10.5KD

Q1:重组蛋白有活性吗?可以做活性检测吗?

活性说明的见说明书bio-activity部分;如果说明书显示没有生物活性或则显示生物活性检测中,则说明这个蛋白没有生物活性数据,需要客户自己检测。

Q2:重组蛋白是否测序验证过?

在售重组蛋白默认不做测序分析/验证。

Q3:蛋白的运输条件是什么?

我们的蛋白有液体和冻干粉2种形式,一般来说冻干粉可以用冰袋、蓝冰、干冰运输;液体可以用蓝冰或干冰运输。

Q4:蛋白是通过什么方式纯化的?

一般通过纯化标签进行亲和纯化,常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。

Q5:蛋白可以用于细胞培养/注射小鼠吗?

内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。

Q6:蛋白发货形式可以是冻干粉吗?可以发液体吗?

默认按照说明书标注的形式发货;如果有特殊需求,需要根据蛋白情况确定。原则上,冻干粉蛋白可以发液体形式,液体蛋白不能发冻干粉形式。

Q7:蛋白Buffer里是否含有denaturants(变性剂)?

我们在售重组蛋白一般不含变性剂,具体见说明书的formulation信息。常见变性剂有:尿素和盐酸胍。

Q8:无标签蛋白纯化方式?

蛋白的纯化方式一般通过标签进行亲和纯化,无标签的蛋白是在纯化后进行了标签切除。

Q9:AF蛋白可以提供吗?

在售的重组蛋白都属于Animal component free protein。在售重组蛋白里不含任何直接来源与动物和人的制品:比如血清,血浆,全血,BSA,OVA等。



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