Recombinant Human Adiponectin/ADIPOQ Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： Q15848
| 别称 |
30 kDa Adipocyte Complement-Related Protein;ACDC;ACRP3;ACRP30;ADIPOQ;Adipocyte;Adipocyte complement-related 30 kDa protein;Adiponectin;Adipose Most Abundant Gene Transcript 1 Protein;C1q and Collagen Domain-Containing Protein;Gelatin-Binding Protein;apM-1
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| 表达系统 |
HEK293 Cells
|
| 序列 |
Glu19-Asn244
|
| 蛋白编码 |
Q15848
|
| 种属 |
Human
|
| 计算分子量 |
25.6 kDa
|
| 表观分子量 |
30 kDa
|
| 标签 |
C-His
|
| 生物活性 |
Not validated for activity
|
| 纯度 |
> 90 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Adiponectin is a secreted protein. It is synthesized exclusively by adipocytes and secreted into plasma. Adiponectin is an important adipokine that is involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Adiponectin Stimulates AMPK phosphorylation and activates in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Adiponectin also antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. It inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. Adiponectin may play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex: LMW, MMW or HMW.
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实验操作视频
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